Malignant glioma is one of the most common primary tumors of the central nervous system. MicroRNAs (miRNAs) play an important role in the occurrence of gliomas, and have a potential value in their treatment. In this study, we investigated the effect of miR-1247-5p on glioma progression. miR-1247-5p showed a low expression in glioma cell lines and glioma tissues from patients. The ectopic overexpression of miR-1247-5p significantly inhibited the proliferation and invasion of U251 cells, induced apoptosis in vitro, and inhibited the growth of transplanted tumor cells in vivo CDC14B was found to be a potential target of miR-1247-5p. The overexpression of miR-1247-5p can significantly down-regulate the expression of CDC14B by directly targeting the 3'UTR of this gene. CDC14B can promote the degradation of p53 and inhibit its activity. The downregulation of CDC14B maintained the activity of p53 and inhibited its degradation. These results suggest that miR-1247-5p functions as a tumor suppressor in human astroglioma U251 cells via the miR-1247-5p/CDC14B/p53 signaling axis. These findings indicate that miR-1247-5p has the potential to be a therapeutic target and diagnostic marker for gliomas.
Keywords: CDC14B; Glioma; Tumor suppressor; miR-1247-5p; p53.
© 2020 by the Association of Clinical Scientists, Inc.