Decreased interleukin(IL)-35 Expression is Associated with Active Intraocular Inflammation in Vogt-Koyanagi-Harada (VKH) Disease

Ocul Immunol Inflamm. 2019;27(4):595-601. doi: 10.1080/09273948.2018.1433306. Epub 2018 Mar 2.

Abstract

Purpose: Recent studies have reported that IL-35 has a protective effect in autoimmune disease. In this study, we explored the role of IL-35 in the pathogenesis of Vogt-Koyanagi-Harada (VKH) disease. Methods: The IL-35/EBI3 and IL-35/P35 mRNA level was assayed by Real-Time PCR. The level of IL-35 in serum was detected by ELISA. PBMCs and monocyte-derived DCs were cultured with or without IL-35 and the concentration of IL-17, IL-10, IFN-γ, IL-6, TNF-α, and IL-1β in supernatants was tested by ELISA. Results: The serum level of IL-35 is reduced in active VKH patients. The mRNA expression of the two subunits IL-35/EBI3 and IL-35/P35 in PBMCs from patients with active VKH was also decreased. IL-35 significantly inhibited IFN-γ and IL-17 expression and induced IL-10 production by PBMCs and inhibited IL-6 production by monocyte-derived DCs. Conclusion: The current study suggests that a decreased IL-35 expression may be involved in the pathogenesis of VKH disease.

Keywords: DCs; IL-35; VKH disease; uceitis.

MeSH terms

  • Adult
  • Cells, Cultured
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Follow-Up Studies
  • Gene Expression Regulation*
  • Humans
  • Interleukins / biosynthesis
  • Interleukins / genetics*
  • Male
  • RNA / genetics*
  • Retrospective Studies
  • Uveitis / etiology
  • Uveitis / genetics*
  • Uveitis / metabolism
  • Uveomeningoencephalitic Syndrome / complications*
  • Uveomeningoencephalitic Syndrome / diagnosis
  • Uveomeningoencephalitic Syndrome / genetics

Substances

  • Interleukins
  • interleukin-35, human
  • RNA