Identification of a novel transcript of human MD2 gene

Chen Shen; A-Dong Shen

doi:10.1016/j.gene.2016.06.030

Identification of a novel transcript of human MD2 gene

Gene. 2016 Sep 15;590(1):123-7. doi: 10.1016/j.gene.2016.06.030. Epub 2016 Jun 16.

Authors

Chen Shen¹, A-Dong Shen²

Affiliations

¹ Key Laboratory of Major Diseases in Children, Ministry of Education, National Key Discipline of Pediatrics (Capital Medical University), National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China.
² Key Laboratory of Major Diseases in Children, Ministry of Education, National Key Discipline of Pediatrics (Capital Medical University), National Clinical Research Center for Respiratory Diseases, Beijing Key Laboratory of Pediatric Respiratory Infection Diseases, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, Beijing 100045, China. Electronic address: shenad16@hotmail.com.

PMID: 27317890
DOI: 10.1016/j.gene.2016.06.030

Abstract

Myeloid differentiation protein 2 (MD2) regulates bacterial lipopolysaccharide (LPS) triggered anti-bacterial immune response as a broker between LPS and Toll-like receptor 4 (TLR4). In this study, we identified a novel naturally occurring spliceosome of human MD2, termed as MD2-T3. This transcript lacked two exons of MD2 gene. By protein structure analysis and literature review, we predicted that MD2-T3 isoform might execute regulatory biological effects such as limiting LPS-triggered TLR4 signaling.

Keywords: Lipopolysaccharide; Myeloid differentiation protein 2; Structure; Transcript.

MeSH terms

Alternative Splicing*
Amino Acid Sequence
Base Sequence
Cell Line
Cloning, Molecular
DNA, Complementary / genetics
DNA, Complementary / immunology
Escherichia coli / genetics
Escherichia coli / metabolism
HeLa Cells
Humans
Lipopolysaccharides / pharmacology
Lymphocyte Antigen 96 / genetics*
Lymphocyte Antigen 96 / immunology
Monocytes / cytology
Monocytes / drug effects
Monocytes / immunology*
Open Reading Frames
Protein Isoforms / genetics
Protein Isoforms / immunology
Protein Structure, Secondary
RNA, Messenger / genetics*
RNA, Messenger / immunology
Recombinant Proteins / genetics
Recombinant Proteins / immunology
Signal Transduction
Spliceosomes / genetics
Spliceosomes / immunology
Toll-Like Receptor 4 / genetics
Toll-Like Receptor 4 / immunology
Transcription, Genetic*

Substances

DNA, Complementary
LY96 protein, human
Lipopolysaccharides
Lymphocyte Antigen 96
Protein Isoforms
RNA, Messenger
Recombinant Proteins
TLR4 protein, human
Toll-Like Receptor 4