Genome scale engineering techniques for metabolic engineering

Metab Eng. 2015 Nov:32:143-154. doi: 10.1016/j.ymben.2015.09.013. Epub 2015 Oct 11.

Abstract

Metabolic engineering has expanded from a focus on designs requiring a small number of genetic modifications to increasingly complex designs driven by advances in genome-scale engineering technologies. Metabolic engineering has been generally defined by the use of iterative cycles of rational genome modifications, strain analysis and characterization, and a synthesis step that fuels additional hypothesis generation. This cycle mirrors the Design-Build-Test-Learn cycle followed throughout various engineering fields that has recently become a defining aspect of synthetic biology. This review will attempt to summarize recent genome-scale design, build, test, and learn technologies and relate their use to a range of metabolic engineering applications.

Keywords: Design-Build-Test-Learn; Genome scale engineering; Metabolic engineering; Synthetic biology.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Animals
  • DNA / genetics
  • Genome / genetics*
  • Humans
  • Metabolic Engineering / methods*
  • Synthetic Biology / trends*

Substances

  • DNA