Abstract
MXD3 is a transcription factor that plays an important role in proliferation of human DAOY medulloblastoma cells. Here, we demonstrate that MXD3 is highly enriched in human precursor B acute lymphoblastic leukemia (preB ALL) samples compared to mobilized peripheral blood mononuclear cells, bone marrow, or hematopoietic stem cells from healthy donors. MXD3 knock-down in the preB ALL cell line Reh resulted in decreased cell numbers with no change in G0/G1, S or G2/M populations but increased apoptosis compared to control cells. Our results suggest that MXD3 is important for survival of Reh preB ALL cells, possibly as an anti-apoptotic factor.
Keywords:
Apoptosis; MXD3; Precursor B acute lymphoblastic leukemia; Proliferation.
Copyright © 2014 Elsevier Inc. All rights reserved.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis / genetics*
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Bone Marrow Cells / cytology
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Bone Marrow Cells / metabolism
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Case-Control Studies
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Cell Cycle Checkpoints / genetics
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Cell Line, Tumor
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Gene Expression Regulation, Neoplastic*
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Hematopoietic Stem Cells / cytology
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Hematopoietic Stem Cells / metabolism
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Humans
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Leukocytes, Mononuclear / cytology
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Leukocytes, Mononuclear / metabolism
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Medulloblastoma / genetics
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Medulloblastoma / metabolism
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Medulloblastoma / pathology
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Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / genetics*
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Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / metabolism
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Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / pathology
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Repressor Proteins / deficiency
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Repressor Proteins / genetics*
Substances
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MXD3 protein, human
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Repressor Proteins