Loss of MXD3 induces apoptosis of Reh human precursor B acute lymphoblastic leukemia cells

Blood Cells Mol Dis. 2015 Apr;54(4):329-35. doi: 10.1016/j.bcmd.2014.12.002. Epub 2014 Dec 20.

Abstract

MXD3 is a transcription factor that plays an important role in proliferation of human DAOY medulloblastoma cells. Here, we demonstrate that MXD3 is highly enriched in human precursor B acute lymphoblastic leukemia (preB ALL) samples compared to mobilized peripheral blood mononuclear cells, bone marrow, or hematopoietic stem cells from healthy donors. MXD3 knock-down in the preB ALL cell line Reh resulted in decreased cell numbers with no change in G0/G1, S or G2/M populations but increased apoptosis compared to control cells. Our results suggest that MXD3 is important for survival of Reh preB ALL cells, possibly as an anti-apoptotic factor.

Keywords: Apoptosis; MXD3; Precursor B acute lymphoblastic leukemia; Proliferation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / genetics*
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / metabolism
  • Case-Control Studies
  • Cell Cycle Checkpoints / genetics
  • Cell Line, Tumor
  • Gene Expression Regulation, Neoplastic*
  • Hematopoietic Stem Cells / cytology
  • Hematopoietic Stem Cells / metabolism
  • Humans
  • Leukocytes, Mononuclear / cytology
  • Leukocytes, Mononuclear / metabolism
  • Medulloblastoma / genetics
  • Medulloblastoma / metabolism
  • Medulloblastoma / pathology
  • Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / genetics*
  • Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / metabolism
  • Precursor B-Cell Lymphoblastic Leukemia-Lymphoma / pathology
  • Repressor Proteins / deficiency
  • Repressor Proteins / genetics*

Substances

  • MXD3 protein, human
  • Repressor Proteins