Abstract
Interchromosomal chimeric RNA molecules are often transcription products from genomic rearrangement in cancerous cells. Here we report the computational detection of an interchromosomal RNA fusion between ZC3HAV1L and CHMP1A from RNA-seq data of normal human mammary epithelial cells, and experimental confirmation of the chimeric transcript in multiple human cells and tissues. Our experimental characterization also detected three variants of the ZC3HAV1L-CHMP1A chimeric RNA, suggesting that these genes are involved in complex splicing. The fusion sequence at the novel exon-exon boundary, and the absence of corresponding DNA rearrangement suggest that this chimeric RNA is likely produced by trans-splicing in human cells.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Cell Line
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Chromosomes, Human, Pair 16 / genetics
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Chromosomes, Human, Pair 7 / genetics
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Computational Biology
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Databases, Genetic
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Endosomal Sorting Complexes Required for Transport / genetics*
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Endosomal Sorting Complexes Required for Transport / metabolism
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Exons
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Gene Fusion*
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Gene Rearrangement
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Humans
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Mutant Chimeric Proteins / genetics*
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RNA, Messenger / genetics
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RNA-Binding Proteins / genetics*
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RNA-Binding Proteins / metabolism
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Real-Time Polymerase Chain Reaction
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Sequence Analysis, DNA
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Trans-Splicing*
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Transcription Factors / genetics*
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Transcription Factors / metabolism
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Vesicular Transport Proteins
Substances
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CHMP1A protein, human
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Endosomal Sorting Complexes Required for Transport
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Mutant Chimeric Proteins
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RNA, Messenger
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RNA-Binding Proteins
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Transcription Factors
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Vesicular Transport Proteins
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ZC3HAV1 protein, human