Dynamin-like protein 1 at the Golgi complex: a novel component of the sorting/targeting machinery en route to the plasma membrane

Exp Cell Res. 2010 Dec 10;316(20):3454-67. doi: 10.1016/j.yexcr.2010.07.020. Epub 2010 Aug 3.

Abstract

The final step in the liberation of secretory vesicles from the trans-Golgi network (TGN) involves the mechanical action of the large GTPase dynamin as well as conserved dynamin-independent fission mechanisms, e.g. mediated by Brefeldin A-dependent ADP-ribosylated substrate (BARS). Another member of the dynamin family is the mammalian dynamin-like protein 1 (DLP1/Drp1) that is known to constrict and tubulate membranes, and to divide mitochondria and peroxisomes. Here, we examined a potential role for DLP1 at the Golgi complex. DLP1 localized to the Golgi complex in some but not all cell lines tested, thus explaining controversial reports on its cellular distribution. After silencing of DLP1, an accumulation of the apical reporter protein YFP-GL-GPI, but not the basolateral reporter VSVG-SP-GFP at the Golgi complex was observed. A reduction in the transport of YFP-GL-GPI to the plasma membrane was confirmed by surface immunoprecipitation and TGN-exit assays. In contrast, YFP-GL-GPI trafficking was not disturbed in cells silenced for BARS, which is involved in basolateral sorting and trafficking of VSVG-SP-GFP in COS-7 cells. Our data indicate a new role for DLP1 at the Golgi complex and thus a role for DLP1 as a novel component of the apical sorting machinery at the TGN is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Oxidoreductases / genetics
  • Animals
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism
  • COS Cells
  • Cell Line, Tumor
  • Cell Membrane / metabolism*
  • Chlorocebus aethiops
  • DNA-Binding Proteins / genetics
  • Dynamins
  • GPI-Linked Proteins / genetics
  • GPI-Linked Proteins / metabolism
  • GTP Phosphohydrolases / genetics
  • GTP Phosphohydrolases / metabolism*
  • Golgi Apparatus / genetics
  • Golgi Apparatus / metabolism*
  • Golgi Apparatus / pathology
  • Humans
  • Luminescent Proteins / genetics
  • Luminescent Proteins / metabolism
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism
  • Microtubule-Associated Proteins / genetics
  • Microtubule-Associated Proteins / metabolism*
  • Mitochondrial Proteins / genetics
  • Mitochondrial Proteins / metabolism
  • Peroxisomes / genetics
  • Peroxisomes / pathology
  • Protein Transport / physiology*
  • RNA, Small Interfering / genetics
  • Rats
  • Transfection
  • Viral Envelope Proteins / genetics
  • Viral Envelope Proteins / metabolism
  • trans-Golgi Network / metabolism

Substances

  • Bacterial Proteins
  • DNA-Binding Proteins
  • G protein, vesicular stomatitis virus
  • GPI-Linked Proteins
  • Luminescent Proteins
  • Membrane Glycoproteins
  • Microtubule-Associated Proteins
  • Mitochondrial Proteins
  • RNA, Small Interfering
  • Viral Envelope Proteins
  • yellow fluorescent protein, Bacteria
  • Alcohol Oxidoreductases
  • C-terminal binding protein
  • GTP Phosphohydrolases
  • DNM1L protein, human
  • Dnm1l protein, rat
  • Dynamins