Identification and characterization of the novel protein CCDC106 that interacts with p53 and promotes its degradation

Jianlin Zhou; Xi Qiao; Ling Xiao; Wei Sun; Lin Wang; Hong Li; Yuan Wu; Xiaofeng Ding; Xiang Hu; Chang Zhou; Jian Zhang

doi:10.1016/j.febslet.2010.02.031

Identification and characterization of the novel protein CCDC106 that interacts with p53 and promotes its degradation

FEBS Lett. 2010 Mar 19;584(6):1085-90. doi: 10.1016/j.febslet.2010.02.031. Epub 2010 Feb 14.

Authors

Jianlin Zhou¹, Xi Qiao, Ling Xiao, Wei Sun, Lin Wang, Hong Li, Yuan Wu, Xiaofeng Ding, Xiang Hu, Chang Zhou, Jian Zhang

Affiliation

¹ Key Laboratory of Protein Chemistry and Developmental Biology of Education Ministry of China, College of Life Science, Hunan Normal University, Changsha, Hunan, China.

PMID: 20159018
DOI: 10.1016/j.febslet.2010.02.031

Abstract

The putative CCDC106 protein was previously identified as a p53-interacting partner by automated yeast two-hybrid screening, but its sequence and function have not been validated experimentally. Here, we identified three variant transcripts of the CCDC106 gene; these transcripts differ in their second exons due to the use of different splice acceptor site, but encode an identical protein of 280 residues. A bipartite nuclear localisation signal at residues 151-164 mediates the nuclear localisation of CCDC106. Double immunofluorescence staining revealed the colocalisation of endogenous CCDC106 and p53 protein in nuclei. The in vivo interaction between CCDC106 and p53 was confirmed by a co-immunoprecipitation assay. Furthermore, we demonstrated that CCDC106 promotes the degradation of p53 protein and inhibits its transactivity.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carrier Proteins / chemistry
Carrier Proteins / genetics
Carrier Proteins / metabolism*
Carrier Proteins / physiology
Cell Nucleus / metabolism
Cloning, Molecular
Green Fluorescent Proteins / metabolism
HeLa Cells
Humans
Nuclear Localization Signals / metabolism
Proteasome Endopeptidase Complex / metabolism
Protein Binding
Protein Isoforms / chemistry
Protein Isoforms / genetics
Protein Isoforms / metabolism
Protein Isoforms / physiology
Protein Processing, Post-Translational* / genetics
RNA, Messenger / isolation & purification
RNA, Messenger / metabolism
Recombinant Fusion Proteins / metabolism
Transcriptional Activation
Tumor Suppressor Protein p53 / metabolism*
Tumor Suppressor Protein p53 / physiology

Substances

CCDC106 protein, human
Carrier Proteins
Nuclear Localization Signals
Protein Isoforms
RNA, Messenger
Recombinant Fusion Proteins
Tumor Suppressor Protein p53
enhanced green fluorescent protein
Green Fluorescent Proteins
Proteasome Endopeptidase Complex