Identification of the CD163 protein domains involved in infection of the porcine reproductive and respiratory syndrome virus

Hanne Van Gorp; Wander Van Breedam; Jan Van Doorsselaere; Peter L Delputte; Hans J Nauwynck

doi:10.1128/JVI.02093-09

Identification of the CD163 protein domains involved in infection of the porcine reproductive and respiratory syndrome virus

J Virol. 2010 Mar;84(6):3101-5. doi: 10.1128/JVI.02093-09. Epub 2009 Dec 23.

Authors

Hanne Van Gorp¹, Wander Van Breedam, Jan Van Doorsselaere, Peter L Delputte, Hans J Nauwynck

Affiliation

¹ Department of Virology, Parasitology and Immunology, Ghent University, 9820 Merelbeke, Belgium.

Abstract

Scavenger receptor CD163 is a key entry mediator for porcine reproductive and respiratory syndrome virus (PRRSV). To identify the CD163 protein domains involved in PRRSV infection, deletion mutants and chimeric mutants were created. Infection experiments revealed that scavenger receptor cysteine-rich (SRCR) domain 5 (SRCR 5) is essential for PRRSV infection, while the four N-terminal SRCR domains and the cytoplasmic tail are not required. The remaining CD163 protein domains need to be present but can be replaced by corresponding SRCR domains from CD163-L1, resulting in reduced (SRCR 6 and interdomain regions) or unchanged (SRCR 7 to SRCR 9) infection efficiency. In addition, CD163-specific antibodies recognizing SRCR 5 are able to reduce PRRSV infection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigens, CD / chemistry*
Antigens, CD / genetics
Antigens, CD / metabolism*
Antigens, Differentiation, Myelomonocytic / chemistry*
Antigens, Differentiation, Myelomonocytic / genetics
Antigens, Differentiation, Myelomonocytic / metabolism*
Humans
Porcine Reproductive and Respiratory Syndrome / metabolism*
Porcine Reproductive and Respiratory Syndrome / virology
Porcine respiratory and reproductive syndrome virus / genetics
Porcine respiratory and reproductive syndrome virus / metabolism*
Porcine respiratory and reproductive syndrome virus / pathogenicity
Protein Isoforms / chemistry
Protein Isoforms / genetics
Protein Isoforms / metabolism
Protein Structure, Tertiary
Receptors, Cell Surface / chemistry*
Receptors, Cell Surface / genetics
Receptors, Cell Surface / metabolism*
Recombinant Fusion Proteins / chemistry
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Scavenger Receptors, Class B / genetics
Scavenger Receptors, Class B / metabolism
Sequence Deletion
Swine
Virus Internalization

Substances

Antigens, CD
Antigens, Differentiation, Myelomonocytic
CD163 antigen
Protein Isoforms
Receptors, Cell Surface
Recombinant Fusion Proteins
Scavenger Receptors, Class B