Abstract
We describe the isolation and characterization of Friend of Prmt1 (Fop), a novel chromatin target of protein arginine methyltransferases. Human Fop is encoded by C1orf77, a gene of previously unknown function. We show that Fop is tightly associated with chromatin, and that it is modified by both asymmetric and symmetric arginine methylation in vivo. Furthermore, Fop plays an important role in the ligand-dependent activation of estrogen receptor target genes, including TFF1 (pS2). Fop depletion results in an almost complete block of estradiol-induced promoter occupancy by the estrogen receptor. Our data indicate that Fop recruitment to the promoter is an early critical event in the activation of estradiol-dependent transcription.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Arginine / metabolism
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Biotinylation
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Cell Line
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Chromatin / metabolism*
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Chromosomal Proteins, Non-Histone / genetics
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Chromosomal Proteins, Non-Histone / metabolism*
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Estradiol / pharmacology
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Humans
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Methylation
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Mice
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Protein Interaction Mapping
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Protein Methyltransferases / metabolism
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Protein-Arginine N-Methyltransferases / genetics
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Protein-Arginine N-Methyltransferases / metabolism*
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Proteomics
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Repressor Proteins / metabolism
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Substrate Specificity
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Transcription Factors
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Transcriptional Activation
Substances
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CHTOP protein, human
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Chromatin
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Chromosomal Proteins, Non-Histone
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Nuclear Proteins
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Repressor Proteins
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Transcription Factors
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Estradiol
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Arginine
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Protein Methyltransferases
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PRMT1 protein, human
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Prmt1 protein, mouse
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Prmt5 protein, mouse
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Protein-Arginine N-Methyltransferases