Inhibition of Bcl10-mediated activation of NF-kappa B by BinCARD, a Bcl10-interacting CARD protein

Ha-Na Woo; Gil-Sun Hong; Joon-Il Jun; Dong-Hyung Cho; Hyun-Woo Choi; Ho-June Lee; Chul-Woong Chung; In-Ki Kim; Dong-Gyu Jo; Jong-Ok Pyo; John Bertin; Yong-Keun Jung

doi:10.1016/j.febslet.2004.10.094

Inhibition of Bcl10-mediated activation of NF-kappa B by BinCARD, a Bcl10-interacting CARD protein

FEBS Lett. 2004 Dec 17;578(3):239-44. doi: 10.1016/j.febslet.2004.10.094.

Authors

Ha-Na Woo¹, Gil-Sun Hong, Joon-Il Jun, Dong-Hyung Cho, Hyun-Woo Choi, Ho-June Lee, Chul-Woong Chung, In-Ki Kim, Dong-Gyu Jo, Jong-Ok Pyo, John Bertin, Yong-Keun Jung

Affiliation

¹ Department of Life Science, Gwangju Institute of Science and Technology, Gwangju 500-712, Republic of Korea.

PMID: 15637807
DOI: 10.1016/j.febslet.2004.10.094

Abstract

We have identified a novel CARD-containing protein from EST database. BinCARD (Bcl10-interacting protein with CARD). BinCARD was ubiquitously expressed. Co-immunoprecipitation, In vitro binding, mammalian two-hybrid, and immunostaining assays revealed that BinCARD interacted with Bcl10 through CARD. BinCARD potently suppressed NF-kappa B activation induced by Bcl10 and decreased the amounts of phosphorylated Bcl10. Mutations at the residue Leu17 or Leu65, which is highly conserved in CARD, abolished the inhibitory effects of BinCARD on both Bcl10-induced activation of NF-kappa B and phosphorylation of Bcl10. Further, expression of BinCARD inhibited Bcl10 phosphorylation induced by T cell activation signal. These results suggest that BinCARD interacts with Bcl10 to inhibit Bcl10-mediated activation of NF-kappa B and to suppress Bcl10 phosphorylation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adaptor Proteins, Signal Transducing / chemistry
Adaptor Proteins, Signal Transducing / genetics
Adaptor Proteins, Signal Transducing / metabolism*
Amino Acid Motifs
Amino Acid Sequence
B-Cell CLL-Lymphoma 10 Protein
Blotting, Western
CARD Signaling Adaptor Proteins / chemistry
CARD Signaling Adaptor Proteins / genetics
CARD Signaling Adaptor Proteins / metabolism*
Carrier Proteins / chemistry
Carrier Proteins / genetics
Carrier Proteins / metabolism*
Cell Line
Conserved Sequence
DNA Mutational Analysis
Down-Regulation
Glutathione Transferase / metabolism
HeLa Cells
Humans
Immunohistochemistry
Jurkat Cells
Leucine / chemistry
Leucine / genetics
Luciferases / metabolism
Lymphocyte Activation
Molecular Sequence Data
Mutagenesis, Site-Directed
NF-kappa B / metabolism*
Phosphorylation
Precipitin Tests
Protein Binding
Protein Structure, Secondary
RNA, Messenger / metabolism
Recombinant Fusion Proteins / isolation & purification
Recombinant Fusion Proteins / metabolism
Sequence Homology, Amino Acid
Signal Transduction
T-Lymphocytes / metabolism
Transcription, Genetic
Two-Hybrid System Techniques

Substances

Adaptor Proteins, Signal Transducing
B-Cell CLL-Lymphoma 10 Protein
BCL10 protein, human
CARD Signaling Adaptor Proteins
CARD19 protein, human
Carrier Proteins
NF-kappa B
RNA, Messenger
Recombinant Fusion Proteins
Luciferases
Glutathione Transferase
Leucine