Time-resolved fluorescence anisotropy imaging applied to live cells

Opt Lett. 2004 Mar 15;29(6):584-6. doi: 10.1364/ol.29.000584.

Abstract

We have developed a wide-field time-resolved imaging system to image quantitatively both the fluorescence lifetime and the rotational correlation time of a fluorophore. Using a polarization-resolved imager, we simultaneously image orthogonal polarization components of the fluorescence emission onto a time-gated intensified CCD. We demonstrate imaging of solvent viscosity variations through the rotational correlation time of fluorescein in a multiwell plate and apply this technique to probe the microviscosity in live cells.

MeSH terms

  • 3T3 Cells
  • Animals
  • Anisotropy*
  • Cell Physiological Phenomena*
  • Dogs
  • Equipment Design
  • Fluorescence*
  • Mice
  • Microscopy, Fluorescence* / instrumentation
  • Microscopy, Polarization* / instrumentation
  • Models, Theoretical
  • Sea Urchins
  • Time Factors
  • Viscosity