Abstract
Bioassay-directed fractionation of a n-hexane extract of Couepia polyandra using an assay to detect inhibitors of the lyase activity of DNA polymerase beta resulted in the isolation of the new triterpene 3beta,16beta,23-triacetoxyolean-12-en-28-oic acid (1) and four known compounds, oleanolic acid, betulinic acid, stigmasterol, and beta-sitosterol. The structure of the new compound was established on the basis of extensive 1D and 2D NMR spectroscopic interpretation. All five compounds inhibited DNA polymerase beta lyase activity.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Base Sequence
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Betulinic Acid
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Chrysobalanaceae / chemistry*
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DNA Polymerase beta / antagonists & inhibitors*
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Enzyme Inhibitors / chemistry
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Enzyme Inhibitors / isolation & purification*
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Enzyme Inhibitors / pharmacology*
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Lyases / antagonists & inhibitors
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Mexico
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Molecular Sequence Data
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Nuclear Magnetic Resonance, Biomolecular
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Oleanolic Acid / analogs & derivatives*
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Oleanolic Acid / chemistry
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Oleanolic Acid / isolation & purification*
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Oleanolic Acid / pharmacology*
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Pentacyclic Triterpenes
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Plant Bark / chemistry
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Plant Stems / chemistry
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Plants, Medicinal / chemistry*
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Triterpenes / chemistry
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Triterpenes / isolation & purification*
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Triterpenes / pharmacology*
Substances
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3beta,16beta,23-triacetoxyolean-12-en-28-oic acid
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Enzyme Inhibitors
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Pentacyclic Triterpenes
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Triterpenes
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oleanane
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Oleanolic Acid
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DNA Polymerase beta
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Lyases
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Betulinic Acid