Identification and cloning of a novel heterogeneous nuclear ribonucleoprotein C-like protein that functions as a transcriptional activator of the hepatitis B virus enhancer II

J Virol. 1992 Dec;66(12):6841-8. doi: 10.1128/JVI.66.12.6841-6848.1992.

Abstract

Liver specificity of hepatitis B virus (HBV) replication has been attributed to the action of its second enhancer (EII). We report here the characterization of EII and the subsequent isolation of a novel liver-specific DNA-binding protein which binds to and activates EII. The cDNA clone of the protein, designated E2BP, was isolated from a lambda gt11 expression library constructed from the hepatoma cell line HuH-6 which was screened with a binding site probe derived from EII. Sequence analysis of E2BP revealed 86.6% homology with a rat heterogeneous nuclear ribonucleoprotein C protein sequence, while conformational studies suggest a helix-loop-helix motif as a DNA-binding site. Cloned E2BP expressed in human fibroblasts by transient transfection displayed EII binding and activating characteristics similar to those of native E2BP in hepatocytes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Blotting, Northern
  • Cell Line
  • Cells, Cultured
  • Chloramphenicol O-Acetyltransferase / genetics
  • Chloramphenicol O-Acetyltransferase / metabolism
  • Cloning, Molecular
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism*
  • Enhancer Elements, Genetic*
  • Hepatitis B virus / genetics*
  • Heterogeneous-Nuclear Ribonucleoproteins
  • Humans
  • Liver / microbiology*
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Open Reading Frames
  • Plasmids
  • Promoter Regions, Genetic
  • RNA, Heterogeneous Nuclear / metabolism
  • RNA, Messenger / genetics
  • RNA, Messenger / isolation & purification
  • RNA, Messenger / metabolism
  • Ribonucleoproteins / genetics*
  • Ribonucleoproteins / metabolism*
  • Sequence Homology, Amino Acid
  • Sequence Homology, Nucleic Acid
  • Trans-Activators / genetics*
  • Trans-Activators / metabolism*
  • Transcription, Genetic*
  • Transfection

Substances

  • DNA-Binding Proteins
  • Heterogeneous-Nuclear Ribonucleoproteins
  • Oligodeoxyribonucleotides
  • RNA, Heterogeneous Nuclear
  • RNA, Messenger
  • Ribonucleoproteins
  • Trans-Activators
  • Chloramphenicol O-Acetyltransferase

Associated data

  • GENBANK/M94630