Abstract
Guanylin is a mammalian peptide homologue of heat-stable enterotoxins that acts on intestinal guanylate cyclase to elicit an increase in cyclic GMP. We have isolated a cDNA encoding an apparent precursor of guanylin from a human intestinal cDNA library. The mRNA is expressed at high levels in human ileum and colon. Human guanylin stimulated increases in T84 cell cyclic GMP levels, displaced 125I-labelled heat-stable enterotoxin (STa) binding to this cell line, and stimulated increases in short-circuit current (Isc) of isolated rat proximal colonic mucosa. This peptide may play a role in regulating fluid and electrolyte absorption in human intestines.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Bacterial Toxins / metabolism
-
Base Sequence
-
Cell Line
-
Chlorides / metabolism
-
Colon / drug effects
-
Colon / metabolism*
-
Colon / physiology
-
Cyclic GMP / metabolism
-
DNA / chemistry
-
DNA / genetics
-
DNA / isolation & purification
-
Electric Conductivity / drug effects
-
Enterotoxins / metabolism
-
Escherichia coli Proteins
-
Gastrointestinal Hormones*
-
Humans
-
Ileum / metabolism*
-
Molecular Sequence Data
-
Natriuretic Peptides
-
Peptide Biosynthesis
-
Peptides / chemistry
-
Peptides / genetics*
-
Peptides / pharmacology*
-
Rats
Substances
-
Bacterial Toxins
-
Chlorides
-
Enterotoxins
-
Escherichia coli Proteins
-
Gastrointestinal Hormones
-
Natriuretic Peptides
-
Peptides
-
heat stable toxin (E coli)
-
guanylin
-
DNA
-
Cyclic GMP
Associated data
-
GENBANK/M97496
-
GENBANK/S39995
-
GENBANK/S39996
-
GENBANK/S40001
-
GENBANK/S40008
-
GENBANK/S40009
-
GENBANK/S40010
-
GENBANK/X58277
-
GENBANK/X66432
-
GENBANK/X70912