Cloning and identification of a novel human ubiquitin-like protein, DC-UbP, from dendritic cells

Biochem Biophys Res Commun. 2003 Jan 17;300(3):800-5. doi: 10.1016/s0006-291x(02)02913-3.

Abstract

Several ubiquitin-like proteins recently discovered have been confirmed to modify proteins akin to ubiquitinization for fine-regulation of intracellular proteins. In the present study, we report a novel ubiquitin-like protein from human dendritic cells (DC), named as dendritic cell-derived ubiquitin-like protein (DC-UbP). The full-length of DC-UbP cDNA is 565bp and encodes 106 amino acids. Ubiquitin domain (UBQ) in DC-UbP shares 28.6% identity and 55% similarity to ubiquitin, but does not possess the conserved C-terminus Gly-Gly of ubiquitin required for ubiquitinization. DC-UbP localized in cytoplasm, especially in mitochondrion, indicating that it may play a role in mitochondrial biology. DC-UbP mRNA was expressed in various tumor cells, but not in adult human normal tissues, suggesting that DC-UbP might be related to tumor genesis. In addition, DC-UbP mRNA expression decreased in the HL60 cells undergoing apoptosis after being stimulated with TRAIL and in the differentiated HL60 cells induced by ARTA. Taken together, DC-UbP might be downregulated during cellular differentiation and apoptosis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Apoptosis / drug effects
  • Apoptosis Regulatory Proteins
  • Base Sequence
  • Blotting, Northern
  • Cell Differentiation / drug effects
  • Cloning, Molecular
  • Dendritic Cells / cytology
  • Dendritic Cells / metabolism*
  • Gene Expression Regulation, Neoplastic / drug effects
  • Humans
  • Membrane Glycoproteins / pharmacology
  • Mitochondria / metabolism
  • Molecular Sequence Data
  • Neoplasms / metabolism*
  • Nerve Tissue Proteins / biosynthesis*
  • Nerve Tissue Proteins / genetics*
  • Organ Specificity
  • RNA, Messenger / biosynthesis
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid
  • Subcellular Fractions / chemistry
  • TNF-Related Apoptosis-Inducing Ligand
  • Tretinoin / pharmacology
  • Tumor Cells, Cultured
  • Tumor Necrosis Factor-alpha / pharmacology
  • Ubiquitin / genetics*
  • Ubiquitins

Substances

  • Apoptosis Regulatory Proteins
  • Membrane Glycoproteins
  • Nerve Tissue Proteins
  • RNA, Messenger
  • TNF-Related Apoptosis-Inducing Ligand
  • TNFSF10 protein, human
  • Tumor Necrosis Factor-alpha
  • UBTD2 protein, human
  • Ubiquitin
  • Ubiquitins
  • Tretinoin

Associated data

  • GENBANK/AF251700