Abstract
Helicobacter pylori adherence in the human gastric mucosa involves specific bacterial adhesins and cognate host receptors. Here, we identify sialyl-dimeric-Lewis x glycosphingolipid as a receptor for H. pylori and show that H. pylori infection induced formation of sialyl-Lewis x antigens in gastric epithelium in humans and in a Rhesus monkey. The corresponding sialic acid-binding adhesin (SabA) was isolated with the "retagging" method, and the underlying sabA gene (JHP662/HP0725) was identified. The ability of many H. pylori strains to adhere to sialylated glycoconjugates expressed during chronic inflammation might thus contribute to virulence and the extraordinary chronicity of H. pylori infection.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adhesins, Bacterial / chemistry
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Adhesins, Bacterial / genetics
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Adhesins, Bacterial / isolation & purification
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Adhesins, Bacterial / metabolism*
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Amino Acid Sequence
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Animals
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Bacterial Adhesion*
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Carbohydrate Sequence
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Carrier Proteins / genetics
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Carrier Proteins / metabolism
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Gastric Mucosa / immunology
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Gastric Mucosa / metabolism
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Gastric Mucosa / microbiology*
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Gastritis / immunology
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Gastritis / metabolism
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Gastritis / microbiology*
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Genes, Bacterial
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Glycoconjugates / metabolism
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Helicobacter Infections / immunology
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Helicobacter Infections / metabolism
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Helicobacter Infections / microbiology*
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Helicobacter pylori / genetics
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Helicobacter pylori / isolation & purification
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Helicobacter pylori / physiology*
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Humans
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Lewis X Antigen / metabolism*
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Macaca mulatta
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Mice
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Mice, Transgenic
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Molecular Sequence Data
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Oligosaccharides / metabolism*
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Sialic Acids / metabolism
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Sialyl Lewis X Antigen
Substances
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Adhesins, Bacterial
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BabA protein, Helicobacter pylori
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Carrier Proteins
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Glycoconjugates
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Lewis X Antigen
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Oligosaccharides
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Sialic Acids
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Sialyl Lewis X Antigen
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galactopyranosyl-1-3-galactopyranosyl-1-3(4)-N-acetylglucosamine