Abstract
The intracellular pathogen Legionella pneumophila subverts vesicle traffic in eukaryotic host cells to create a vacuole that supports replication. The dot/icm genes encode a protein secretion apparatus that L. pneumophila require for biogenesis of this vacuole. Here we show that L. pneumophila produce a protein called RalF that functions as an exchange factor for the ADP ribosylation factor (ARF) family of guanosine triphosphatases (GTPases). The RalF protein is required for the localization of ARF on phagosomes containing L. pneumophila. Translocation of RalF protein through the phagosomal membrane is a dot/icm-dependent process. Thus, RalF is a substrate of the Dot/Icm secretion apparatus.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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ADP-Ribosylation Factor 1 / genetics
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ADP-Ribosylation Factor 1 / metabolism*
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ADP-Ribosylation Factors / metabolism
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Acanthamoeba / microbiology
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Amino Acid Sequence
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Animals
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Carrier Proteins / genetics
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Carrier Proteins / metabolism
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Cell Line
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Genes, Bacterial
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Guanine Nucleotide Exchange Factors / chemistry
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Guanine Nucleotide Exchange Factors / genetics
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Guanine Nucleotide Exchange Factors / metabolism*
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Humans
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Legionella / genetics
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Legionella pneumophila / genetics
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Legionella pneumophila / growth & development
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Legionella pneumophila / metabolism*
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Macrophages / microbiology
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Mice
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Mice, Inbred A
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Molecular Sequence Data
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Phagosomes / metabolism*
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Phagosomes / microbiology*
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Protein Structure, Tertiary
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Protein Transport
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RNA, Bacterial / genetics
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RNA, Bacterial / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Recombinant Fusion Proteins / metabolism
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Sequence Homology, Amino Acid
Substances
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Bacterial Proteins
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Carrier Proteins
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Guanine Nucleotide Exchange Factors
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RNA, Bacterial
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RNA, Messenger
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Recombinant Fusion Proteins
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ADP-Ribosylation Factor 1
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ADP-Ribosylation Factors