Abstract
Haemophilus influenzae utilizes hemoglobin and hemoglobin-haptoglobin as heme sources. The H. influenzae hemoglobin- and hemoglobin-haptoglobin binding protein genes, hgpA, hgpB, and hgpC, contain lengths of tetrameric CCAA repeats. Using an hgpA-lacZ translational gene fusion, we demonstrate phase-variable expression of lacZ associated with alteration in the length of the CCAA repeat region.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacterial Outer Membrane Proteins / biosynthesis
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Bacterial Outer Membrane Proteins / genetics*
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Bacterial Proteins*
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Base Sequence
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Carrier Proteins / biosynthesis
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Carrier Proteins / genetics*
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DNA Primers
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Gene Expression Regulation, Bacterial*
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Genes, Bacterial
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Haemophilus influenzae / genetics*
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Haptoglobins / metabolism*
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Hemoglobins / metabolism*
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Microsatellite Repeats / genetics*
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Molecular Sequence Data
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Protein Biosynthesis
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Recombinant Fusion Proteins / biosynthesis
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Restriction Mapping
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beta-Galactosidase / genetics
Substances
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Bacterial Outer Membrane Proteins
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Bacterial Proteins
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Carrier Proteins
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DNA Primers
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Haptoglobins
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Hemoglobins
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HgpC protein, Haemophilus influenzae
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Recombinant Fusion Proteins
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haptoglobin-hemoglobin complex
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hemoglobin-binding protein, bacteria
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beta-Galactosidase