PMC

The UV absorption spectrum of taurine mono-bromamine (TauBr) λ_max = 288 nm.

BAT inhibits nuclear the translocation of phosphorylated NF-κB p65 subunit. Immunofluorescence of phosphorylated NF-κB p65 subunit (Ser 536) in the LPS-stimulated J774.A1 Mφs after (0.1-0.5 mM) BAT or 100 mM taurine treatment for 1.5 h. The phosphorylated NF-κB p65 was detected by Alexa Fluor 488-labeled immunostaining (green); nuclei were stained with Hoechst (blue). Untreated cells were used as a negative control (NC), while cells treated only with LPS were used as a positive control for an inflammatory response (x630 magnification). In particular, the groups of J774.A1 Mφς were the following: Negative control (NC), (100 ng/ml) LPS-treated J774.A1 Mφs (LPS group), (100 ng/ml) LPS plus 0.1 mM BAT-treated J774.A1 Mφs (LPS + 0.1 mM BAT group), (100 ng/ml) LPS plus 0.3 mM BAT-treated J774.A1 Mφs (LPS + 0.3 mM BAT group), (100 ng/ml) LPS plus 0.5 mM BAT-treated J774.A1 Mφs (LPS + 0.5 mM BAT group), (100 ng/ml) LPS plus 100 mM taurine-treated J774.A1 Mφs (LPS + 100 mM taurine group). Mφς, macrophages; LPS, lipopolysaccharide; BAT, bromamine T.

pH effect on Tau-NHBr reactivity. (a) Tau-NHBr (5 mM) was prepared in water using a 100-fold excess of taurine. The solution was diluted to 0.25 mM of NaH₂PO₄/Na₂HPO₄ (50 mM) solutions combined to reach the indicated pH; (b,c) Reactivity of Tau-NHBr with dansylglycine at pH 5.0 and 9.0.

Bromamine T (BAT) is cytotoxic on a wide spectrum of cancer cells. The following cells: (A) RKO, (B) Caco2, (C) HT-29 (D) MDA-MB-231, (E) MDA-MB-468, (F) HeLa, (G) WM-164 cells were treated with (0.5–10 mΜ) BAT or 0.166 mM cisplatin (CIS) for 24–72 h. The percentage of viable cells was assessed upon BAT or Tau treatment versus negative control (NC) using the Crystal Violet procedure and statistical analysis was performed. ns: not significant, * p < 0.05. ** p < 0.01. *** p < 0.001.**** p < 0.0001.