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Antagonist activity at cADPR binding protein in sea urchin egg homogenate assessed as inhibition of cADPR-induced Ca2+ release by Fluo-3 based fluorescence assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed Citation
Agonist activity at cADPR binding protein in sea urchin egg homogenate assessed as increase in Ca2+ release by Fluo-3 based fluorescence assay
Assay data:2 Active, 1 Activity ≤ 1 µM, 2 Tested
Antagonist activity at cADPR binding protein in sea urchin egg homogenate assessed as decrease in cADPR-induced Ca2+ release at 23 uM by Fluo-3 based fluorescence assay
Assay data:1 Tested
SummaryPubMed Citation
Agonist activity at cADPR binding protein in sea urchin egg homogenate assessed as increase in Ca2+ release at 100 uM by Fluo-3 based fluorescence assay relative to control
Agonist activity at cADPR binding protein in sea urchin egg homogenate assessed as increase in Ca2+ release at lowest concentration by Fluo-3 based fluorescence assay
Desensitization of NAADP receptor in sea urchin egg homogenate assessed as ability to induce time dependent sub-threshold desensitization of calcium release pretreated for 7 mins followed by 1 uM NAADP added by measuring inhibition of calcium release by fluorometric assay
Assay data:3 Active, 1 Activity ≤ 1 nM, 3 Activity ≤ 1 µM, 3 Tested
Agonist activity at NAADP receptor in sea urchin egg homogenate assessed as increase in calcium release by fluo-3 based flurometric assay
Assay data:3 Active, 2 Activity ≤ 1 µM, 3 Tested
Desensitization of NAADP receptor in Strongylocentrotus purpuratus egg homogenates assessed as inhibition of NAADP-induced Ca2+ release pre-treated for 7 mins before NAADP addition by Fluo-3 dye based fluorometric assay
Assay data:11 Active, 2 Activity ≤ 1 nM, 10 Activity ≤ 1 µM, 12 Tested
Displacement of [32P]NAADP from NAADP receptor in Strongylocentrotus purpuratus egg homogenates incubated for 90 mins by liquid scintillation counting method
Assay data:11 Active, 2 Activity ≤ 1 nM, 9 Activity ≤ 1 µM, 12 Tested
Activation of NAADP receptor in Strongylocentrotus purpuratus egg homogenates assessed as Ca2+ release by Fluo-3 dye based fluorometric assay
Assay data:7 Active, 5 Activity ≤ 1 µM, 12 Tested
Ratio of activation of NAADP receptor in Strongylocentrotus purpuratus egg homogenates by Ca2+ release assay to IC50 for desensitization of NAADP receptor in Strongylocentrotus purpuratus egg homogenates by Ca2+ release assay
Ratio of activation of NAADP receptor in Strongylocentrotus purpuratus egg homogenates by Ca2+ release assay to IC50 for NAADP receptor in Strongylocentrotus purpuratus egg homogenates by [32P]NAADP binding assay
Desensitization of NAADP receptor in Strongylocentrotus purpuratus egg homogenates assessed as inhibition of NAADP-induced Ca2+ release pre-treated for 7 mins before NAADP addition by Fluo-3 dye based fluorometric assay relative to NAADP
Assay data:11 Tested
Displacement of [32P]NAADP from NAADP receptor in Strongylocentrotus purpuratus egg homogenates incubated for 90 mins by liquid scintillation counting method relative to NAADP
Activation of NAADP receptor in Strongylocentrotus purpuratus egg homogenates assessed as Ca2+ release by Fluo-3 dye based fluorometric assay relative to NAADP
Inhibition of bipolar spindle formation in Strongylocentrotus purpuratus eggs
Assay data:2 Tested
Displacement of [32P]NAADP from NAADP receptor in sea urchin microsomes after 90 mins by liquid scintillation counting
Assay data:9 Active, 9 Activity ≤ 1 µM, 10 Tested
Agonist activity at NAADP receptor in sea urchin egg homogenate assessed as induction of Ca2+ release by Fluo-3 staining based fluorimetric assay
Assay data:6 Active, 4 Activity ≤ 1 µM, 10 Tested
Binding affinity to NAADP receptor in sea urchin microsomes assessed as fold increase in IC50 for displacement of [32P]NAADP from receptor relative to NAADP
Assay data:9 Tested
Agonist activity at NAADP receptor in sea urchin egg homogenate assessed as desensitization of NAADP-induced Ca2+ release at => 1 nM treated for 9 mins before NAADP challenge by Fluo-3 staining based fluorimetric assay
Assay data:9 Active, 9 Tested
SummaryCompounds, ActivePubMed Citation
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