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Inhibition of Campylobacter jejuni PgIC expressed in Escherichia coli by affinity chromatography
Assay data:1 Tested
SummaryPubMed Citation
Inhibition of Campylobacter jejuni DHDPS using using ASA as a substrate in presence of NADPH by DHDPS-DHDPR coupled assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed Citation
Inhibition of Campylobacter jejuni phosphoglycosyl transferase Pg1D
Reversible inhibition of Campylobacter jejuni dihydrodipicolinate synthase
SummaryCompounds, ActiveCompounds, activity ≤ 1 µM
Inhibition of Campylobacter jejuni dihydrodipicolinate synthase
Assay data:1 Active, 1 Activity ≤ 1 µM, 2 Tested
Competitive inhibition of Campylobacter jejuni NCTC 11168 PglD acetyltransferase expressed in Escherichia coli BL-21(DE3) strain (Stratagene) assessed as release of CoASH preincubated for 30 mins followed by addition of UDP-4-aminosugar and 5 fold excess AcCoA as substrate by Ellman's method
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of Campylobacter jejuni NCTC 11168 PglD acetyltransferase expressed in Escherichia coli BL-21(DE3) strain (Stratagene) assessed as release of CoASH preincubated for 30 mins followed by addition of 300 uM AcCoA and 500 uM UDP-4-aminosugar as substrate by Ellman's method
Assay data:39 Active, 20 Activity ≤ 1 µM, 39 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of Campylobacter jejuni NCTC 11168 PglD acetyltransferase expressed in Escherichia coli BL-21(DE3) strain (Stratagene) assessed as release of CoASH preincubated for 45 mins followed by addition of AcCoA and UDP-4-aminosugar measured after 30 mins by fragment-based high-throughput screening analysis
Assay data:1 Active, 1 Activity ≤ 1 µM, 4 Tested
Competitive inhibition of Campylobacter jejuni NCTC 11168 PglD acetyltransferase expressed in Escherichia coli BL-21(DE3) strain (Stratagene) assessed as release of CoASH preincubated for 30 mins followed by addition of UDP-4-aminosugar and 2 fold excess AcCoA as substrate by Ellman's method
Effect on quaternary structure of Campylobacter jejuni NCTC 11168 PglD acetyltransferase expressed in Escherichia coli BL-21(DE3) strain (Stratagene) assessed as polydispersity index of PgID at 250 uM by dynamic light scattering method (Rvb = 31%)
SummaryPubMed CitationRelated BioAssays by Target
Effect on quaternary structure of Campylobacter jejuni NCTC 11168 PglD acetyltransferase expressed in Escherichia coli BL-21(DE3) strain (Stratagene) assessed as hydrodynamic radius of apo PgID at 250 uM by dynamic light scattering method (Rvb = 34 Angstrom)
Competitive inhibition of Campylobacter jejuni NCTC 11168 PglD acetyltransferase expressed in Escherichia coli BL-21(DE3) strain (Stratagene) assessed as release of CoASH preincubated for 30 mins followed by addition of AcCoA and higher levels of UDP-4-aminosugar as substrate by Ellman's method
Binding affinity to Campylobacter jejuni NCTC 11168 PglD acetyltransferase expressed in Escherichia coli BL-21(DE3) strain (Stratagene) assessed as change in melting temperature at 100 uM by Sypro orange dye-based differential scanning fluorimetry
Assay data:39 Tested
Antibacterial activity against Campylobacter jejuni after 17 hrs by NCCLS broth microdilution method
Antimicrobial activity against Campylobacter jejuni ATCC 33291 incubated at 37 degC for 24 hrs by broth macrodilution method
Assay data:3 Tested
Antibacterial activity against Campylobacter jejuni NCTC 11168 assessed as growth inhibition by broth dilution method
Assay data:22 Tested
Antibacterial activity against Campylobacter jejuni JB201 infected in chicken assessed as decrease in bacterial colonization at 50 mg/L
SummaryCompounds, ActivePubMed Citation
Antibacterial activity against Cj1211-negative Campylobacter jejuni harboring aphA3 and tet(O) genes
Assay data:2 Tested
Antibacterial activity against Cj1211-positive Campylobacter jejuni harboring aphA3 and tet(O) genes
Antimicrobial activity against tylosin pre-exposed compound-resistant Campylobacter jejuni
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