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Growth inhibition of Lactococcus lactis NZ9000 transfected with Streptococcus agalactiae COH1 NSR S236A mutant incubated for 5 hrs by spectrophotometry
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed Citation
Growth inhibition of Lactococcus lactis NZ9000 transfected with Streptococcus agalactiae COH1 NSR incubated for 5 hrs by spectrophotometry
Growth inhibition of Lactococcus lactis NZ9000 transfected with pNZ-Erm plasmid incubated for 5 hrs by spectrophotometry
Antimicrobial activity against Lactococcus lactis after 18 to 24 hrs in aerobic condition by two-fold serial dilutions method
Assay data:3 Tested
SummaryPubMed Citation
Inhibition of Lactococcus lactis Nis1 harboring in Lactococcus lactis NZ9000 assessed as decrease in Nis1 induced resistance measured as increase in growth inhibition incubated for 5 hrs by optical density based plate reader assay
Assay data:4 Active, 4 Activity ≤ 1 µM, 4 Tested
Inhibition of Lactococcus lactis ATP-binding cassette transporter NisFEG harboring in Lactococcus lactis NZ9000 assessed as decrease in NisFEG induced resistance measured as increase in growth inhibition incubated for 5 hrs by optical density based plate reader assay
Growth inhibition of nisin-sensitive Lactococcus lactis NZ9000Cm incubated for 5 hrs by optical density based plate reader assay
Induction of pore formation in Lactococcus lactis NZ9000 transfected with Streptococcus agalactiae COH1 NSR assessed as increase in florescence intensity at 30 nM measured after 100 secs by sytox green-based fluorescence assay
Assay data:1 Active, 2 Tested
SummaryCompounds, ActivePubMed Citation
Resistance index, ratio of IC50 for growth inhibition of Lactococcus lactis NZ9000 transfected with Streptococcus agalactiae COH1 NSR S236A mutant incubated for 5 hrs by spectrophotometry to IC50 for growth inhibition of Lactococcus lactis NZ9000 transfected with pNZ-Erm plasmid incubated for 5 hrs by spectrophotometry
Assay data:2 Tested
Resistance index, ratio of IC50 for growth inhibition of Lactococcus lactis NZ9000 transfected with Streptococcus agalactiae COH1 NSR incubated for 5 hrs by spectrophotometry to IC50 for growth inhibition of Lactococcus lactis NZ9000 transfected with pNZ-Erm plasmid incubated for 5 hrs by spectrophotometry
Bactericidal activity against Lactococcus lactis in aerobic condition
Assay data:3 Active, 3 Tested
Growth inhibition of Lactococcus lactis NZ9000 transfected with Streptococcus agalactiae NSR at 300 uM incubated for 5 hrs in absence of nisin by optical density based assay
Assay data:1 Tested
Induction of pore formation in Lactococcus lactis NZ9000Cm assessed as increase in florescence intensity at 3 times antibacterial IC50 measured after 200 secs by sytox green-based fluorescence assay
Assay data:2 Active, 3 Tested
Induction of pore formation in Lactococcus lactis NZ9000Cm assessed as increase in florescence intensity at 3 times antibacterial IC50 measured after 200 secs by sytox green-based fluorescence assay relative to control
Antimicrobial activity against Streptococcus lactis assessed as zone of inhibition at 10 ug/ml
Assay data:4 Tested
Binding affinity to wild-type Lactococcus lactis MG1363 N-terminal His8-tagged ThiT expressed in Lactococcus lactis NZ9000 by isothermal calorimetric titration method
Assay data:2 Active, 4 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Binding affinity to Lactococcus lactis MG1363 N-terminal His8-tagged ThiT His125Ala mutant expressed in Lactococcus lactis NZ9000 by isothermal calorimetric titration method
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Binding affinity to Lactococcus lactis MG1363 N-terminal His8-tagged ThiT His125Asn mutant expressed in Lactococcus lactis NZ9000 by isothermal calorimetric titration method
Binding affinity to Lactococcus lactis MG1363 N-terminal His8-tagged ThiT Glu84Ala mutant expressed in Lactococcus lactis NZ9000 by isothermal calorimetric titration method
Assay data:1 Active, 1 Tested
Binding affinity to Lactococcus lactis MG1363 N-terminal His8-tagged ThiT Glu84Gln mutant expressed in Lactococcus lactis NZ9000 by isothermal calorimetric titration method
SummaryPubMed CitationRelated BioAssays by Target
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