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Decrease in MAPK expression in human MCF7 cells at 50 ug/ml measured after 30 mins in presence of CCL2 by Western blot analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed Citation
Antiinvasive activity against human MCF7 cells assessed as inhibition of cell invasion at 50 ug/ml measured after 24 hrs in presence of CCL2 by crystal violet staining based Boyden chamber analysis
Antimigratory activity against human MCF7 cells assessed as reduction in cell migration at 10 to 50 ug/ml measured after 5 hrs in presence of CCL2 by wound healing assay relative to control
Assay data:2 Tested
SummaryPubMed Citation
Anti-proliferative activity against human MDA-MB-231 cells assessed as reduction in cell proliferation at 10 to 50 ug/ml compound pretreated with CCL2 followed by incubation with cells and measured after 48 hrs by Sulforhodamine B staining based WST assay
Assay data:1 Tested
Anti-proliferative activity against human MCF7 cells assessed as reduction in cell proliferation at 10 to 50 ug/ml compound pretreated with CCL2 followed by incubation with cells and measured after 48 hrs by Sulforhodamine B staining based WST assay
Induction of cell cycle arrest in human MCF7 cells assessed as accumulation of cells at G2/M phase incubated for 72 hrs in presence of CCL2 by PI staining based flow cytometric analysis
Binding affinity to human CXCL10 assessed as dissociation constant incubated for 3 mins by surface plasmon resonance analysis
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to human CXCL8 assessed as off-rate constant incubated for 3 mins by surface plasmon resonance analysis
Binding affinity to human CXCL8 assessed as on-rate constant incubated for 3 mins by surface plasmon resonance analysis
Binding affinity to human CXCL12 assessed as dissociation constant incubated for 3 mins by surface plasmon resonance analysis
Binding affinity to human CXCL8 assessed as dissociation constant incubated for 3 mins by surface plasmon resonance analysis
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Binding affinity to human CCL5 assessed as off-rate constant incubated for 3 mins by surface plasmon resonance analysis
Binding affinity to human CCL5 assessed as on-rate constant incubated for 3 mins by surface plasmon resonance analysis
Binding affinity to human CCL5 assessed as dissociation constant incubated for 3 mins by surface plasmon resonance analysis
Binding affinity to human CCL2 assessed as off-rate constant incubated for 3 mins by surface plasmon resonance analysis
Binding affinity to human CCL2 assessed as on-rate constant incubated for 3 mins by surface plasmon resonance analysis
Binding affinity to human CCL2 assessed as dissociation constant incubated for 3 mins by surface plasmon resonance analysis
Binding affinity to human CCL2 at 100 uM by glycan microarray analysis
Assay data:12 Tested
Binding affinity to human CXCL12 at 100 uM by glycan microarray analysis
Binding affinity to human CCL21 at 100 uM by glycan microarray analysis
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