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Binding affinity to human partial length BAZ2A (M1792 to L1905 residues) expressed in bacterial expression system by BROMOscan assay
Assay data:4 Tested
SummaryPubMed CitationRelated BioAssays by Target
Selectivity interaction (Bromodomain panel (BLI assay)) EUB0000267b BAZ2A
Assay data:1 Tested
SummaryRelated BioAssays by Target
Selectivity interaction (ITC) EUB0000222b BAZ2A
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Selectivity interaction (Biolayer Interferometry experiment (Octet-RED BLI)) EUB0000222b BAZ2A
Selectivity interaction (Bromodomain panel (DSF assay)) EUB0000222b BAZ2A
Selectivity interaction (Bromodomain panel (DSF assay)) EUB0000192d BAZ2A
Affinity Biochemical interaction (AlphaScreen assay) EUB0000222b BAZ2A
Affinity Biochemical interaction (Isothermal titration calorimetry (ITC)) EUB0000192d BAZ2A
Affinity On-target Cellular interaction (Chemoproteomic competition binding assay (endogenous full-length BAZ2A from HuT78 extracts)) EUB0000267b BAZ2A
Affinity On-target Cellular interaction (Chemoproteomic competition binding assay (endogenous full-length BAZ2A from HuT78 extracts)) EUB0000222b BAZ2A
Assay data:1 Active, 1 Tested
SummaryCompounds, ActiveRelated BioAssays by Target
Affinity On-target Cellular interaction (FRAP assay (utilizing GFP-tagged BAZ2A full length protein transfected into U2OS cells)) EUB0000192d BAZ2A
Affinity Phenotypic Cellular interaction (FRAP assay (displacement of BAZ2A/B from chromatin in SAHA-treated human U2OS cells, transfected with GFP-BAZ2A fusion construct)) EUB0000267b BAZ2A
Affinity Phenotypic Cellular interaction (FRAP assay (displacement of BAZ2A/B from chromatin in SAHA-treated human U2OS cells, transfected with GFP-BAZ2A fusion construct)) EUB0000222b BAZ2A
Binding affinity to His6-tagged BAZ2A (unknown origin) (1796 to 1899 residues) by isothermal titration calorimetry assay
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of His6-tagged BAZ2A (unknown origin) (1796 to 1899 residues) using H3K(Ac)14 substrate by AlphaScreen assay
Assay data:1 Active, 59 Tested
Inhibition of His6-tagged BAZ2A (unknown origin) (1796 to 1899 residues) assessed as residual activity at 500 uM using H3K(Ac)14 substrate by AlphaScreen assay relative to control
Assay data:8 Tested
Inhibition of His6-tagged BAZ2A (unknown origin) (1796 to 1899 residues) assessed as residual activity at 100 uM using H3K(Ac)14 substrate by AlphaScreen assay relative to control
Assay data:12 Tested
Binding affinity to human BAZ2A bromodomain assessed as residual activity at 300 nM by BROMOscan method relative to control
BAZ2A DiscoverX BROMOscan
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