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Inhibition of human NTPDase1
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of recombinant soluble human C-terminal His6-tagged NTPDase1 (Thr38 to Val478 residues) expressed in CHO cells using ATP as substrate measured after 30 mins by capillary electrophoresis
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of human CD39 expressed in CHO cells incubated for 4 hrs before addition of AMP and further incubated for 60 mins by colorimetric assay
Inhibition of human NTPDase 1 expressed in human COS7 cells using ATP as substrate at 50 uM incubated for 5 mins followed by substrate addition and measured after 30 mins by capillary electrophoresis method
Inhibition of mouse CD39
Assay data:2 Active, 1 Activity ≤ 1 µM, 4 Tested
Inhibition of rat CD39 expressed in CHO cells using ATP as substrate incubated for 10 mins by UV absorbance method
Assay data:1 Active, 1 Activity ≤ 1 µM, 3 Tested
Inhibition of mouse CD39 expressed in green monkey Cos-7 cells using ATP as substrate preincubated for 3 mins followed by substrate addition by malachite green reagent based assay
Assay data:2 Tested
Inhibition of human CD39 expressed in green monkey Cos-7 cells using ATP as substrate preincubated for 3 mins followed by substrate addition by malachite green reagent based assay
Assay data:1 Active, 2 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of human CD39 using ATP as substrate preincubated for 3 mins followed by substrate addition and measured after 10 to 15 mins by Malachite green reagent based assay
Assay data:1 Active, 3 Tested
Inhibition of human CD39 using ATP as substrate preincubated for 3 mins followed by substrate addition and measured after 10 to 15 mins by Cheng-Prusoff analysis
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
Inhibition of human CD39 using ATP as substrate preincubated for 3 mins followed by substrate addition and measured after 10 to 15 mins by Dixon and Cornish-Bowden plot analysis
Competitive inhibition of human CD39 using ATP as substrate preincubated for 3 mins followed by substrate addition and measured after 15 mins by Dixon and Cornish-Bowden plot analysis
Inhibition of CD39 (unknown origin)
Assay data:3 Activity ≤ 1 µM, 3 Tested
SummaryCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of human CD39 expressed in CHO cells using ATP as substrate preincubated for 1 hr followed by substrate addition and measured after 50 mins by colorimetric assay
Inhibition of human NTPdase-1 expressed in COS7 cell membranes at 100 uM using ATP as substrate preincubated for 3 mins followed by susbtrate addition and measured after 10 mins by malachite green dye based method
Assay data:4 Active, 6 Tested
Inhibition of human NTPDase1 expressed in African green monkey COS7 cell membrane fraction using ATP as substrate preincubated for 10 mins followed by substrate addition measured after 10 mins by malachite green reagent-based assay
Assay data:14 Tested
Inhibition of rat NTPDase1 expressed in CHO cells using ATP as substrate preincubated for 10 mins followed by substrate addition measured after 10 mins by malachite green reagent-based assay
Assay data:5 Active, 20 Tested
Activation of human NTPDase1 using ATP as substrate preincubated for 3 mins followed by substrate addition measured after 10 mins by malachite green reagent-based assay relative to control
Drug metabolism assessed as human NTPDase1-mediated compound hydrolysis at 100 uM relative to ATP
Assay data:4 Tested
Inhibition of human NTPDase1 at 100 uM using ATP as substrate preincubated for 3 mins followed by substrate addition measured after 10 mins by malachite green reagent-based assay
Assay data:3 Tested
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