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Inhibition of human recombinant hexokinase-1 assessed as NADPH generation measured after 1 hr by HPLC analysis
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to hexokinase-1 in human MCF7 cell lysate measured after overnight incubation by LC-MS/MS analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Binding affinity to hexokinase-1 in human MCF7 cell lysate assessed as HK-1 pull down by western blotting based Co-immunoprecipitation assay
Non competitive inhibition of recombinant human HK2 expressed in Escherichia coli BL21(DE3) assessed as decrease in Vmax using glucose as substrate by Lineweaver-Burk double reciprocal plot analysis
Non competitive inhibition of recombinant human HK2 expressed in Escherichia coli BL21(DE3) assessed as decrease in Vmax using ATP as substrate by Lineweaver-Burk double reciprocal plot analysis
Binding affinity to recombinant human HK2 expressed in Escherichia coli BL21(DE3) assessed as dissociation constant measured after 15 mins by microscale thermophoresis assay
Inhibition of HK2 mouse RAW264.7 cells assessed as reduction in LPS-induced glycolysis at 50 uM followed by LPS-stimulation and measured after 4 hrs by seahorse XFe96 analyser based assay
Non competitive inhibition of recombinant human HK2 expressed in Escherichia coli BL21(DE3) assessed as Km using 12.5 uM glucose as substrate by Lineweaver-Burk double reciprocal plot analysis (Rvb = 0.64 mM)
Non competitive inhibition of recombinant human HK2 expressed in Escherichia coli BL21(DE3) assessed as Km using 6.25 uM glucose as substrate by Lineweaver-Burk double reciprocal plot analysis (Rvb = 0.64 mM)
Non competitive inhibition of recombinant human HK2 expressed in Escherichia coli BL21(DE3) assessed as Km using 3.125 uM glucose as substrate by Lineweaver-Burk double reciprocal plot analysis (Rvb = 0.64 mM)
Non competitive inhibition of recombinant human HK2 expressed in Escherichia coli BL21(DE3) assessed as Vmax using 12.5 uM glucose as substrate by Lineweaver-Burk double reciprocal plot analysis (Rvb = 0.012 OD/min)
Non competitive inhibition of recombinant human HK2 expressed in Escherichia coli BL21(DE3) assessed as Vmax using 6.25 uM glucose as substrate by Lineweaver-Burk double reciprocal plot analysis (Rvb = 0.012 OD/min)
Non competitive inhibition of recombinant human HK2 expressed in Escherichia coli BL21(DE3) assessed as Vmax using 3.125 uM glucose as substrate by Lineweaver-Burk double reciprocal plot analysis (Rvb = 0.012 OD/min)
Non competitive inhibition of recombinant human HK2 expressed in Escherichia coli BL21(DE3) assessed as Km using 12.5 uM ATP as substrate by Lineweaver-Burk double reciprocal plot analysis (Rvb = 1.62 mM)
Non competitive inhibition of recombinant human HK2 expressed in Escherichia coli BL21(DE3) assessed as Km using 6.25 uM ATP as substrate by Lineweaver-Burk double reciprocal plot analysis (Rvb = 1.62 mM)
Non competitive inhibition of recombinant human HK2 expressed in Escherichia coli BL21(DE3) assessed as Km using 3.125 uM ATP as substrate by Lineweaver-Burk double reciprocal plot analysis (Rvb = 1.62 mM)
Non competitive inhibition of recombinant human HK2 expressed in Escherichia coli BL21(DE3) assessed as Vmax using 12.5 uM ATP as substrate by Lineweaver-Burk double reciprocal plot analysis (Rvb = 0.019 OD/min)
Non competitive inhibition of recombinant human HK2 expressed in Escherichia coli BL21(DE3) assessed as Vmax using 6.25 uM ATP as substrate by Lineweaver-Burk double reciprocal plot analysis (Rvb = 0.019 OD/min)
Non competitive inhibition of recombinant human HK2 expressed in Escherichia coli BL21(DE3) assessed as Vmax using 3.125 uM ATP as substrate by Lineweaver-Burk double reciprocal plot analysis (Rvb = 0.019 OD/min)
Inhibition of recombinant human HK2 expressed in Escherichia coli BL21(DE3) using glucose as substrate preincubated for 10 mins followed by substrate addition in presence of ATP by G6PDH/NADP coupled assay
Assay data:2 Active, 21 Tested
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