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RNAi screen for vemurafenib enhancer genes in BRAFV600 melanoma - Primary Screen
Assay data:790 Active, 18119 Tested
Summary
Binding affinity to immobilized recombinant human His-tagged p47phox SH3A-B domain (151 to 285 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as maximal response by SPR assay relative to 2-methyl-4-piperazin-1-yl-quinoline
Assay data:9 Tested
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to immobilized recombinant human His-tagged p47phox SH3A-B domain (151 to 285 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as maximal response by SPR assay relative to quinolin-2-amine
Assay data:25 Tested
Binding affinity to immobilized recombinant human His-tagged p47phox SH3A-B domain (151 to 285 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as dissociation constant by SPR assay
Assay data:37 Tested
Binding affinity to recombinant human His-tagged p47phox SH3-AB domain (151 to 285 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as change in melting temperature at 2 mM incubated for 10 to 15 mins by SYPRO orange dye based thermal shift assay
Assay data:8 Tested
Binding affinity to recombinant human His-tagged p47phox SH3A-B domain (151 to 285 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as change in melting temperature at 1 mM incubated for 10 to 15 mins by SYPRO orange dye based thermal shift assay
Binding affinity to recombinant human His-tagged p47phox SH3A-B domain (151 to 285 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as change in melting temperature at 0.5 mM incubated for 10 to 15 mins by SYPRO orange dye based thermal shift assay
Binding affinity to recombinant human His-tagged p47phox SH3A-B domain (151 to 285 residues) expressed in Escherichia coli BL21 (DE3) cells assessed as change in melting temperature at 0.25 mM incubated for 10 to 15 mins by SYPRO orange dye based thermal shift assay
Covalent inhibition of recombinant human His-tagged p47phox SH3A-B domain (151 to 285 residues) expressed in Escherichia coli BL21 (DE3) cells interaction with Cy5-p22phox149-162 incubated for 2 hrs by fluorescence polarization competition assay
Assay data:1 Tested
Ratio of Ki for recombinant human His-tagged p47phox SH3A-B domain (151 to 285 residues) expressed in Escherichia coli BL21 (DE3) cells in presence of TCEP to Ki for recombinant human His-tagged p47phox SH3A-B domain (151 to 285 residues) expressed in Escherichia coli BL21 (DE3) cells by fluorescence polarization competition assay
Covalent inhibition of recombinant human His-tagged p47phox SH3A-B domain (151 to 285 residues) expressed in Escherichia coli BL21 (DE3) cells interaction with Cy5-p22phox149-162 incubated for 10 mins in presence of TCEP by fluorescence polarization competition assay
Covalent inhibition of recombinant human His-tagged p47phox SH3A-B domain (151 to 285 residues) expressed in Escherichia coli BL21 (DE3) cells interaction with Cy5-p22phox149-162 incubated for 10 mins by fluorescence polarization competition assay
Assay data:74 Tested
Inhibition of recombinant human His-tagged p47phox SH3A-B domain (151 to 285 residues) expressed in Escherichia coli BL21 (DE3) cells interaction with Cy5-p22phox149-162 in presence of TritonX-100 by fluorescence polarization competition assay
Assay data:3 Active, 3 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Genome-wide siRNA screen of genes regulating the Lipopolysaccharide-induced TNF-alpha response in human macrophages_Primary screen TNF readout
Assay data:2853 Active, 18113 Tested
SummaryRelated BioAssays by Same Project
A screen to identify synthetic lethal interactions with E-cadherin in an MCF10A isogenic cell line
Assay data:2442 Active, 18052 Tested
SummaryPubMed Citation
A screen to identify genes that regulate lymphatic endothelial cell migration - Primary Screen
Assay data:1034 Active, 18120 Tested
SummaryRelated BioAssays by Depositor
Identification of proteins required for the processing of the EWS-FLI1 nascent transcript
Assay data:59220 Tested
A whole genome RNAi screen highlights components of the ER/Golgi as a prominent source of resistance to immunotoxin-mediated cytotoxicity
Assay data:555 Active, 64752 Tested
RNAi screen for vorinostat resistance genes - Primary Screen
Assay data:450 Active, 18118 Tested
Genome-wide high-content siRNA screens for regulators of Parkin translocation in selective mitophagy (Ambion Library)
Assay data:9227 Active, 64752 Tested
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