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WNK1 SGC Tm panel (DSF)
Assay data:1 Tested
SummaryRelated BioAssays by Target
Thermal Shift Assay. Domain: start/stop: Q194-E488
Assay data:27 Tested
Inhibition of WNK1 (unknown origin) at 10 uM relative to control
Assay data:2 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of WNK1 in human MDA-MB-231 cells assessed as reduction in OSR1 phosphorylation measured after 24 hrs by Western blot analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of TEV cleavage site-fused His6-tagged WNK1 (194 to 483 residues) (unknown origin) expressed in Rosetta (DE3) cells using GST-OSR1(314 to 344) peptide as substrate measured after 30 mins by [gamma-32P]-ATP based liquid scintillation counting analysis
Assay data:5 Active, 6 Tested
Inhibition of TEV cleavage site-fused His6-tagged WNK1 (194 to 483 residues) (unknown origin) expressed in Rosetta (DE3) cells using GST-OSR1(314 to 344) peptide as substrate incubated for 2 hrs by kinase-glo luminescence assay
Assay data:1 Active, 13 Tested
Inhibition of human WNK1 (198 to 401 residues) using biotinylated STK3 as substrate incubated for 2 hrs by HTRF assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
WNK1 DiscoveRx kinase panel
WNK1(h) Kinase panel
Inhibition of WNK1 (unknown origin) at 1 uM
Inhibition of human recombinant WNK1 assessed as reduction in substrate phosphorylation at 500 nM using ATP and Ulight-CKKSRGDYMTMQIG (IRS-1) incubated for 60 min by LANCE detection method
WNK In Vitro Radiometric Assays from Article 10.1021/acschembio.6b00511: "Discovery and Characterization of Allosteric WNK Kinase Inhibitors."
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
WNK1 HTRF Assay from Article 10.1021/acschembio.6b00511: "Discovery and Characterization of Allosteric WNK Kinase Inhibitors."
Assay data:1 Active, 2 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
WNK1 Mobility Shift Assay from Article 10.1021/acschembio.6b00511: "Discovery and Characterization of Allosteric WNK Kinase Inhibitors."
In vitro OSR1 Phosphorylation Assay (OSR1) from Article 10.1038/nchembio.2168: "Small-molecule WNK inhibition regulates cardiovascular and renal function."
In vitro MBP Phosphorylation Assay (MBP) from Article 10.1038/nchembio.2168: "Small-molecule WNK inhibition regulates cardiovascular and renal function."
Assay data:2 Active, 1 Activity ≤ 1 nM, 2 Activity ≤ 1 µM, 2 Tested
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