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Selectivity interaction (Methyltransferase panel (Scintillation proximity assay (SPA))) EUB0000212b NSD3
Assay data:1 Tested
SummaryRelated BioAssays by Target
Selectivity interaction (Methyltransferase panel) EUB0000198b NSD3
Selectivity interaction (Protein methyltransferase panel (activity assay)) EUB0000211b NSD3
Inhibition of human NSD3 at 10 uM by radioisotope-based filter binding assay
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of N-terminal GST tagged NSD3 (1021 to 1322 residues) (unknown origin) expressed in Escherichia coli at 1 uM using 3H-SAM as a substrate relative to control
Inhibition of GST-tagged NSD3-PWWP1 domain (247 to 398 residues) (unknown origin) by TR-FRET assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Binding affinity to NSD3 (unknown origin) assessed as dissociation constant
Inhibition of human recombinant N-terminal GST-tagged NSD3 (1021 to 1322 residues) expressed in Escherichia coli at 50 uM using DNA as substrate by scintillation/filter assay relative to control
Inhibition of human recombinant N-terminal GST-tagged NSD3 (1021 to 1322 residues) expressed in Escherichia coli at 10 uM using DNA as substrate by scintillation/filter assay
Inhibition of NSD3 (unknown origin) at 1 to 10 uM
Assay data:2 Tested
HMT Assay from Article 10.1038/nchembio.2304: "An allosteric PRC2 inhibitor targeting the H3K27me3 binding pocket of EED."
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by DepositorRelated BioAssays by Target
Inhibition of human NSD3-SET using histone H3.1 as substrate incubated for 1 hr by Western blot analysis
Displacement of Halo-tagged Histone H3.3 from Nanoluc-tagged NSD3- PWWP1 (unknown origin) transfected in human U2OS cells at 3 to 50 uM preincubated for one day followed by Nano-GloR substrate addition at the next day and measured withing 10 mins by NanoBRET assay
Binding affinity to NSD3 PWWP1 domain (unknown origin) assessed as aggregation temperature at 400 uM by heating at 25 to 85 degC by differential static light scattering method
Binding affinity to NSD3 PWWP1 domain (263 to 398 residues) (unknown origin) expressed in Escherichia coli BL21(DE3) by SPR method
Assay data:1 Active, 1 Activity ≤ 1 µM, 2 Tested
Inhibition of NSD3 (unknown origin) using biotin-labeled histone substrate assessed as residual activity at 50 uM in presence of [3H-Me]SAM by scintillation proximity assay relative to control
Assay data:24 Tested
Inhibition of NSD3 (unknown origin) at <5 uM using histone H3 peptide (1 to 45) as substrate in presence of co-factor SAM by LC-MS analysis
Inhibition of human NSD3 up to 50 uM using chicken nucleosome as substrate and in presence of [3H]-SAM incubated for 1 hr by filter-based assay
Inhibition of NSD3 (unknown origin) at 10 uM using [3H-SAM] and nucleosome as substrate incubated for 1 hr by scintillation proximity assay
RNAi screen for vemurafenib enhancer genes in BRAFV600 melanoma - Primary Screen
Assay data:790 Active, 18119 Tested
Summary
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