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Inhibition of recombinant TET2 in human Hep3B cells assessed as reduction of 5hmC formation at 50 uM incubated for 48 hrs in presence of CuSO4 by HPLC-ESI-MS/MS
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of recombinant TET2 in human Hep3B cells assessed as reduction of 5hmC levels at 50 uM incubated for 48 hrs in presence of CuSO4 by dot blotting analysis
Inhibition of recombinant human TET2 catalytic domain assessed as reduction of 5hmC formation at 125 uM using hemi-methylated synthetic oligomer 5'-AATTAGAA[5meC]GCTCCTT-3' as substrate incubated for 30 mins in presence of CuSO4 by LC-MS method
Inhibition of recombinant human TET2 catalytic domain assessed as reduction of 5hmC formation at 125 uM using hemi-methylated synthetic oligomer 5'-AATTAGAA[5meC]GCTCCTT-3' as substrate incubated for 30 mins by LC-MS method
Assay data:2 Active, 2 Tested
Inhibition of N-terminal His6-tagged TET2 (unknown origin) expressed in Escherichia coli assessed as reduction in hmC and fC levels preincubated for 30 mins followed by Fe(II) addition and measured after 1 hrs using 5'-CACmCGGTG-3' palindromic oligonucleotide as substrate by MALDI-TOF MS analysis
Assay data:3 Active, 18 Tested
Inhibition of N-terminal His6-tagged TET2 (unknown origin) expressed in Escherichia coli assessed as reduction in 5-methylcytosine oxidation preincubated for 30 mins followed by Fe(II) addition for 1 hrs and Msp1 addition and measured after 4 hrs using oligonucleotide as substrate by FRET assay
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of N-terminal His6-tagged TET2 (unknown origin) expressed in Escherichia coli assessed as reduction in 5-methylcytosine oxidation by measuring 5-carboxylcytosine level by FRET assay
Assay data:2 Tested
Reversible inhibition of recombinant human GST-tagged TET2 (1099 to 1936 residues) expressed in Escherichia coli BL21 (DE3)pLysS assessed as restoration of enzyme activity in presence of alpha-KG at 20 uM incubated for 2 hrs by ELISA
Binding affinity to human recombinant TET2 (1099 to 1936) catalytic domain assessed as dissociation constant by micro-scale thermophoresis analysis
Assay data:4 Active, 2 Activity ≤ 1 µM, 4 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Potency index, ratio of IC50 of NOG to IC50 of compound for inhibition of recombinant human GST-tagged TET2 (1099 to 1936 residues) expressed in Escherichia coli BL21 (DE3)pLysS assessed as reduction in 5hmc level incubated for 2 hrs by ELISA
Assay data:3 Tested
Inhibition of recombinant human GST-tagged TET2 (1099 to 1936 residues) expressed in Escherichia coli BL21 (DE3)pLysS assessed as reduction in 5hmc level incubated for 2 hrs by ELISA
Assay data:1 Active, 8 Tested
Inhibition of TET2 (unknown origin) at 50 mM in presence of alpha-KG relative to control
Inhibition of TET2 (unknown origin) at 10 mM in presence of alpha-KG relative to control
Inhibition of TET2 (unknown origin)
Assay data:1 Active, 2 Tested
Inhibition of recombinant human C-terminal His/N-terminal FLAG-tagged TET2 (1129 to 2002 residues) expressed in baculovirus infected sf9 cells assessed as reduction in oxidation of methylated dsDNA after 2 hrs by chemiluminescence assay
Inhibition of recombinant human C-terminal His/N-terminal FLAG-tagged TET2 (1129 to 2002 residues) expressed in baculovirus infected sf9 cells assessed as reduction in oxidation of methylated dsDNA at 100 uM after 2 hrs by chemiluminescence assay relative to control
Assay data:1 Active, 6 Tested
RNAi screen for vemurafenib enhancer genes in BRAFV600 melanoma - Primary Screen
Assay data:790 Active, 18119 Tested
Summary
Genome-wide siRNA screen of genes regulating the Lipopolysaccharide-induced TNF-alpha response in human macrophages_Primary screen TNF readout
Assay data:2853 Active, 18113 Tested
SummaryRelated BioAssays by Same Project
A screen to identify synthetic lethal interactions with E-cadherin in an MCF10A isogenic cell line
Assay data:2442 Active, 18052 Tested
SummaryPubMed Citation
A screen to identify genes that regulate lymphatic endothelial cell migration - Primary Screen
Assay data:1034 Active, 18120 Tested
SummaryRelated BioAssays by Depositor
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