Warning: The NCBI web site requires JavaScript to function. more...
An official website of the United States government
The .gov means it's official. Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you're on a federal government site.
The site is secure. The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.
Covalent inhibition of CDK12 (unknown origin) using histone H1 as substrate incubated for 10 mins in presence of [gamma-32P]ATP by radioactivity based kinase assay
Assay data:1 Active, 4 Activity ≤ 1 µM, 4 Tested
SummaryRelated BioAssays by Target
Inhibition of CDK12 (unknown origin)
Assay data:1 Tested
Inhibition of CDK (unknown origin)
Inhibition of CDK12 (unknown origin) incubated for 120 mins in presence of ATP by HotSpot kinase assay
Assay data:2 Active, 1 Activity ≤ 1 µM, 2 Tested
Inhibition of recombinant N-terminal GST/His6-fusion tagged human CDK12/Cyclin K expressed in Sf9 insect cells incubated for 20 mins followed by [33P]gamma-ATP addition and measured after 2 hrs by radiometric HotSpot Kinase assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of recombinant CDK12 (unknown origin) expressed in baculovirus infected insect cells using Pol II CTD peptide as substrate in presence of [gamma-32P]ATP by radiometric kinase assay
Binding affinity to partial length human wild-type CDK12 expressed in mammalian expression system assessed as dissociation constant by DiscoverX KINOMEscan assay
Binding affinity to CDK12 (unknown origin) assessed as dissociation constant
Inhibition of recombinant CDK12 (unknown origin) by radiometric kinase assay
Inhibition of CDK12/cyclin K (unknown origin) preincubated for 10 mins followed by ATP and substrate addition and measured after 60 mins by HTRF analysis
Inhibition of GST-tagged human CDK12/Cyclin K expressed in Sf9 cells using RBER-IRStide peptide as substrate in presence of ATP and [gamma33-P] ATP by radioisotope filter binding assay
Assay data:7 Active, 7 Activity ≤ 1 µM, 7 Tested
Protac activity at CRBN/CDK12 in human MDA-MB-231 cells assessed as inhibition of degradation of CDK12 at 500 nM measured after 6 hrs in presence of thalidomide
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Protac activity at CRBN/CDK12 in human MDA-MB-231 cells assessed as degradation of CDK12 at 500 nM measured after 6 hrs in presence of bafilomycin by Western blot analysis
SummaryPubMed CitationRelated BioAssays by Target
Protac activity at CRBN/CDK12 in human MDA-MB-231 cells assessed as degradation of CDK12 at 500 nM measured after 6 hrs in presence of carfilzomib by Western blot analysis
Protac activity at CRBN/CDK12 in human MDA-MB-231 cells assessed as induction of degradation at 500 nM incubated upto 24 hrs by Western blotting analysis
Protac activity at CRBN/CDK12 in human MFM-223 cells assessed as induction of degradation at 500 nM incubated upto 24 hrs by Western blotting analysis
Protac activity at CRBN/CDK12 in human MDA-MB-231 cells assessed as induction of degradation by Western blotting analysis
Protac activity at CRBN/CDK12 in human MFM-223 cells assessed as induction of degradation by Western blotting analysis
Protac activity at CRBN/CDK12 in human MDA-MB-231 cells assessed as induction of CDK12 degradation at 1 uM incubated for 15 hrs by immunoblotting analysis relative to control
Assay data:27 Tested
Binding affinity to human CDK12 (715 to 1052 residues)/CyclinK (1 to 267 residues) expressed in baculovirus infected in Sf9 cells by Biolayer interferometry
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
Filters: Manage Filters
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on