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Inhibition of catalytic activity of LRS in HEK293 cells assessed as reduction in mTORC1 activation by measuring leucine-induced S6K phosphorylation at 12.5 to 200 uM by immunoblotting analysis
Assay data:3 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of catalytic activity of LRS (unknown origin) by aminoleucylation assay
Assay data:3 Active, 3 Activity ≤ 1 µM, 3 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of Leucyl-tRNA synthetase-mediated mTORC1 signaling activation in human HEK293 cells assessed as reduction in leucine-induced S6K phosphorylation at 200 uM preincubated for 50 mins followed by leucine-stimulation and measured after 10 mins by Western blot analysis
Assay data:21 Active, 48 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of Leucyl-tRNA synthetase-mediated mTORC1 signaling activation in human HEK293 cells assessed as reduction in leucine-induced S6K phosphorylation preincubated for 50 mins followed by leucine-stimulation and measured after 10 mins by Western blot analysis
Assay data:2 Tested
Inhibition of human leucyl-tRNA synthetase
RNAi screen for vemurafenib enhancer genes in BRAFV600 melanoma - Primary Screen
Assay data:790 Active, 18119 Tested
Summary
Inhibition of LRS canonical enzyme activity in HEK293 cells assessed as effect on enzyme-mediated aminoleucylation
Inhibition of LRS-dependent mTORC1 activation in HEK293 cells assessed as reduction in leucine-induced mTORC1-mediated S6 Kinase phosphorylation at 20 to 200 uM pretreated before leucine stimulation measured after 10 mins by immunoblot analysis
Assay data:1 Tested
Inhibition of LRS-dependent mTORC1 activation in HEK293 cells assessed as reduction in leucine-induced mTORC1-mediated S6 Kinase phosphorylation pretreated before leucine stimulation measured after 10 mins by immunoblot analysis
Inhibition of LRS-dependent mTORC1 activation in HEK293 cells assessed as reduction in leucine-induced mTORC1-mediated S6 Kinase phosphorylation at 200 uM pretreated before leucine stimulation measured after 10 mins by immunoblot analysis
Assay data:5 Active, 7 Tested
Inhibition of LRS-dependent mTORC1 activation in HEK293 cells assessed as reduction in leucine-induced mTORC1-mediated S6 Kinase phosphorylation at 100 nM pretreated before leucine stimulation measured after 10 mins by immunoblot analysis
Assay data:1 Active, 1 Tested
Inhibition of cytoplasmic LeuRS in human HepG2 cells assessed as reduction in protein synthesis preincubated for 48 hrs followed by L-[14C]leucine addition measured after 3 hrs by liquid scintillation counting analysis
Assay data:8 Tested
Inhibition of human N-terminal 6His-tagged cytoplasmic LeuRS expressed in Escherichia coli BL21(DE3) assessed as L-[14C]leucine incorporation in to Escherichia coli tRNA after 20 mins by liquid scintillation counting analysis
Inhibition of LRS aminooleucylation in HEK293 cells assessed as residual activity
Inhibition of LRS-mediated mTORC1 activation in HEK293 cells assessed as decrease in leucine-induced S6K phosphorylation at T389 at 100 uM treated prior to leucine induction measured after 10 mins by Western blot method
Assay data:1 Active, 2 Tested
Inhibition of LRS-mediated mTORC1 activation in HEK293 cells assessed as decrease in leucine-induced S6K phosphorylation at T389 treated prior to leucine induction measured after 10 mins by Western blot method
Inhibition of LRS-mediated mTORC1 activation in HEK293 cells assessed as decrease in leucine-induced S6K phosphorylation at T389 at 200 uM treated prior to leucine induction measured after 10 mins by Western blot method
Assay data:5 Active, 9 Tested
Inhibition of LRS-mediated mTORC1 activation in HEK293 cells assessed as decrease in leucine-induced S6K phosphorylation at T389 at 800 uM treated prior to leucine induction measured after 10 mins by Western blot method
Inhibition of LRS-mediated mTORC1 activation in HEK293 cells assessed as decrease in leucine-induced S6K phosphorylation at T389 at 100 nM treated prior to leucine induction measured after 10 mins by Western blot method
Inhibition of human cytoplasmic LeuRS assessed as reduction in protein synthesis measured after 10 mins in presence of [3H]Leu by scintillation counting method
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