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Activity at recombinant human N-terminal rat ST3 receptor-fused TAS2R10 expressed in HEK293T cells co-expressing Galpha16gust44 assessed as effect on strychnine-induced intracellular calcium level at 100 uM by FLIPR assay
Assay data:2 Tested
SummaryRelated BioAssays by Target
Agonist activity at recombinant human N-terminal rat ST3 receptor-fused TAS2R10 expressed in HEK293T cells co-expressing Galpha16gust44 assessed as effect on intracellular calcium level by FLIPR assay
Assay data:1 Tested
Agonist activity at recombinant human N-terminal rat ST3 receptor-fused TAS2R10 expressed in HEK293T cells co-expressing Galpha16gust44 assessed as effect on intracellular calcium level at 100 uM by FLIPR assay
Assay data:2 Active, 2 Tested
SummaryCompounds, ActiveRelated BioAssays by Target
Agonist activity at human TAS2R10 expressed in HEK293T cells co-expressing Galpha15 assessed as increase in intracellular calcium level at 20 mM by Calcium-3 dye based fluorescence assay relative to control
Assay data:3 Tested
Agonist activity at human TAS2R10 expressed in HEK293T cells co-expressing Galpha15 assessed as increase in intracellular calcium level by Calcium-3 dye based fluorescence assay
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMRelated BioAssays by Target
Activity at human TAS2R10 expressed in HEK-293T-Galpha16-gustducin44 cells assessed as effect on calcium response by FLIPR assay
Activity at human TAS2R10 expressed in HEK-293T-Galpha16-gustducin44 cells assessed as threshold concentration of compound at which point receptor detects compounds by measuring calcium response by FLIPR assay
Assay data:4 Active, 4 Tested
Activity at human TAS2R10 expressed in HEK293/15 cells assessed as compound concentration showing threshold of activation on calcium response by FLIPR assay
Assay data:2 Active, 1 Activity ≤ 1 µM, 2 Tested
Activity at human TAS2R10 expressed in HEK293/15 cells assessed as effect on calcium response by FLIPR assay
Competitive-antagonist activity at recombinant human N-terminal rat ST3 receptor-fused TAS2R10 expressed in HEK293T cells co-expressing Galpha16gust44 assessed as inhibition of strychnine-induced increase in intracellular calcium level by measuring maximum signal amplitude at 100 uM by FLIPR assay (Rvb = 0.42 +/- 0.09 a.u.)
Competitive-antagonist activity at recombinant human N-terminal rat ST3 receptor-fused TAS2R10 expressed in HEK293T cells co-expressing Galpha16gust44 assessed as inhibition of strychnine-induced increase in intracellular calcium level by measuring maximum signal amplitude at 30 uM by FLIPR assay (Rvb = 0.42 +/- 0.09 a.u.)
Competitive-antagonist activity at recombinant human N-terminal rat ST3 receptor-fused TAS2R10 expressed in HEK293T cells co-expressing Galpha16gust44 assessed as inhibition of strychnine-induced increase in intracellular calcium level by measuring maximum signal amplitude at 10 uM by FLIPR assay (Rvb = 0.42 +/- 0.09 a.u.)
Competitive-antagonist activity at recombinant human N-terminal rat ST3 receptor-fused TAS2R10 expressed in HEK293T cells co-expressing Galpha16gust44 assessed as inhibition of strychnine-induced increase in intracellular calcium level by measuring strychnine EC50 at 100 uM by FLIPR assay (Rvb = 0.39 +/- 0.09 uM)
Competitive-antagonist activity at recombinant human N-terminal rat ST3 receptor-fused TAS2R10 expressed in HEK293T cells co-expressing Galpha16gust44 assessed as inhibition of strychnine-induced increase in intracellular calcium level by measuring strychnine EC50 at 30 uM by FLIPR assay (Rvb = 0.39 +/- 0.09 uM)
Assay data:1 Active, 1 Tested
Competitive-antagonist activity at recombinant human N-terminal rat ST3 receptor-fused TAS2R10 expressed in HEK293T cells co-expressing Galpha16gust44 assessed as inhibition of strychnine-induced increase in intracellular calcium level by measuring strychnine EC50 at 10 uM by FLIPR assay (Rvb = 0.39 +/- 0.09 uM)
Antagonist activity at recombinant human N-terminal rat ST3 receptor-fused TAS2R10 expressed in HEK293T cells co-expressing Galpha16gust44 assessed as inhibition of strychnine-induced increase in intracellular calcium level at 100 uM by FLIPR assay
Agonist activity at recombinant human N-terminal rat ST3 receptor-fused TAS2R10 expressed in HEK293T cells co-expressing Galpha16gust44 assessed as increase in intracellular calcium level by FLIPR assay
Agonist activity at recombinant human N-terminal rat ST3 receptor-fused TAS2R10 expressed in HEK293T cells co-expressing Galpha16gust44 assessed as increase in intracellular calcium level at 100 uM by FLIPR assay
Activation of full-length human TAS2R10 expressed in HEK293T/Galpha16-gustducin 44 cells assessed as effect on calcium flux by measuring deltaF/F0 ratio by Fluo-4 dye based FLIPR assay
Activation of full-length human TAS2R10 expressed in HEK293T/Galpha16-gustducin 44 cells assessed as effect on calcium flux by measuring deltaF/F0 ratio at 0.3906 mM by Fluo-4 dye based FLIPR assay
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