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Inhibition of human Myc-DDK-tagged IDH1 R132C mutant expressed in human U87MG cells assessed as reduction in 2-HG levels after 24 hrs by RapidFire high-throughput mass spectrometry assay
Assay data:4 Active, 3 Activity ≤ 1 µM, 4 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of wild type IDH1 (unknown origin) homodimer assessed as reduction in NADPH consumption using alpha-ketoglutarate as substrate preincubated with enzyme for 1 to 16 hrs followed by substrate addition and measured after 1 hr by Diaphorase/Resazurin dye based fluorescence assay
Assay data:1 Tested
SummaryPubMed CitationRelated BioAssays by Target
Inhibition of IDH1 R132H mutant (unknown origin) expressed in HEK293T cells using alpha-KG in presence of NADPH by LC-MS/MS analysis
Inhibition of wild type IDH1 (unknown origin) using isocitrate as substrate preincubated for 30 mins followed by substrate addition in presence of NADP+ measured up to 5 mins by resorufin-based fluorescence assay
Assay data:21 Tested
Competitive inhibition of recombinant human IDH1 R132H mutant expressed in Escherichia coli using NADPH as cofactor at 5 to 20 uM by Lineweaver-Burk plot analysis in presence of alpha-KG
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Competitive inhibition of IDH1 R132H mutant using alpha-ketoglutarate as substrate by stopped-flow spectrophotometric analysis
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