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Binding affinity to full-length N-terminal 6His-tagged HMGB1 (unknown origin) by NMR spectra
Assay data:3 Tested
SummaryPubMed CitationRelated BioAssays by Target
Binding affinity to 6His-tagged HMGB1 BoxB (unknown origin) incubated for 15 mins by MST analysis
Assay data:1 Tested
Binding affinity to 6His-tagged HMGB1 BoxA (unknown origin) incubated for 15 mins by MST analysis
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Binding affinity to 6His-tagged HMGB1 (unknown origin) incubated for 15 mins by MST analysis
Assay data:6 Active, 2 Activity ≤ 1 µM, 13 Tested
Inhibition of HMGB1 (unknown origin)-induced cell permeability in HUVEC cells at 100 uM preincubated for 3 hrs followed by HMGB-1 addition and measured after 16 hrs by Evans blue dye based assay relative to control
Assay data:13 Tested
SummaryRelated BioAssays by Target
Inhibition of HMGB1 (unknown origin)-induced cell permeability in HUVEC cells at 100 nM preincubated for 3 hrs followed by HMGB-1 addition and measured after 16 hrs by Evans blue dye based assay relative to control
Assay data:30 Tested
Inhibition of HMGB1 (unknown origin) at 10 uM by ELISA relative to control
RNAi screen for vemurafenib enhancer genes in BRAFV600 melanoma - Primary Screen
Assay data:790 Active, 18119 Tested
Summary
Binding affinity to recombinant HMGB1 box B (unknown origin)
Assay data:2 Active, 2 Activity ≤ 1 µM, 2 Tested
Binding affinity to recombinant HMGB1 box A (unknown origin)
Binding affinity to N-terminal 6His-tagged HMGB1 AI region (1 to 87 residues) (unknown origin) expressed in Escherichia coli JM109 by SPR assay
Binding affinity to N-terminal 6His-tagged HMGB1 Bj region (88 to 181 residues) (unknown origin) expressed in Escherichia coli JM109 by SPR assay
Binding affinity to recombinant HMGB1 A box region (1 to 89 residues) (unknown origin) expressed Escherichia coli after 5 mins by fluorescence-based assay
Binding affinity to recombinant HMGB1 B box region (90 to 175 residues) (unknown origin) expressed Escherichia coli after 5 mins by fluorescence-based assay
Inhibition of ICM-BP binding to recombinant human HMGB1 assessed as reduction in Cy5-azide incorporation at 1 to 20 uM pretreated for 60 mins followed by ICM-BP addition for 20 mins and subsequent UV light irradiation for 5 mins by fluorescence assay
Inhibition of ICM-BP binding to recombinant human HMGB1 assessed as reduction in Cy5-azide incorporation pretreated for 60 mins followed by ICM-BP addition for 20 mins and subsequent UV light irradiation for 5 mins by fluorescence assay
Inhibition of HMGB1-mediated human neutrophil migration in HUVEC after 6 hrs by crystal violet staining based microscopy
Inhibition of HMGB1-mediated human neutrophil adhesion in HUVEC after 6 hrs by ELISA
Inhibition of HMGB1-mediated E-selectin expression level in HUVEC at 0.1 to 1 uM after 6 hrs by ELISA
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of HMGB1-mediated ICAM-1 expression level in HUVEC at 0.1 to 1 uM after 6 hrs by ELISA
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