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Inhibition of eIF4G-induced full length recombinant human N-terminal His6/SUMO-tagged eIF4A1 RNA dependent ATPase activity expressed in Escherichia coli BL21(DE3) using single stranded poly(U) RNA as substrate after 30 mins by ADP-Glo luminescence assay
Assay data:5 Tested
SummaryPubMed CitationRelated BioAssays by Target
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SummaryPubMed Citation
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Inhibition of e1F4A1 (unknown origin) in rabbit reticulocyte assessed as capped-dependent translation incubated for 1 hr by dual glo luciferase assay
Assay data:5 Active, 5 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Binding affinity to EIF4A1 in HEK293 proteomes assessed as spectral count ratio at 20 uM incubated for 16 hrs by TAMRA biotin-azide dye based LC-MS/MS analysis relative to control
Assay data:1 Tested
Inhibition of eIF4G-induced full length recombinant human N-terminal His6/SUMO-tagged eIF4A1 RNA dependent ATPase activity expressed in Escherichia coli BL21(DE3) using single stranded poly(U) RNA as substrate after 40 mins by ADP-Glo luminescence assay
Assay data:1 Active, 4 Tested
RNAi screen for vorinostat resistance genes - Tertiary Screen (multiple cell lines)
Assay data:105 Active, 106 Tested
Inhibition of elF4G-induced full length recombinant human N-terminal His6-SUMO-tagged eIF4A1 RNA dependent ATPase activity expressed in Escherichia coli BL21(DE3) using single stranded poly(U) RNA as substrate after 30 mins by ADP-Glo luminescence assay
Assay data:4 Tested
Inhibition of eIF4A1 (unknown origin) expressed in Escherichia coli BL21(DE3) at 0.01 to 100 uM using Poly(U) RNA as substrate preincubated for 10 mins followed by substrate addition measured after 60 mins by malachite green dye-based assay
Assay data:2 Active, 2 Tested
Inhibition of eIF4A1 (unknown origin) expressed in Escherichia coli BL21(DE3) using Poly(U) RNA as substrate preincubated for 10 mins followed by substrate addition measured after 60 mins by malachite green dye-based assay
Assay data:1 Active, 2 Tested
Inhibition of eIF4A (unknown origin) expressed in Escherichia coli BL21(DE3) at 100 uM preincubated for 10 mins followed by ATP and fluorescently-labeled RNA addition measured after 10 mins by real-time fluorescence assay relative to control
Inhibition of eIF4A (unknown origin) expressed in Escherichia coli BL21(DE3) at 100 uM preincubated for 10 mins followed by ATP and fluorescently-labeled RNA addition measured after 10 mins by real-time fluorescence assay
Inhibition of eIF4G-induced full-length human recombinant His6-tagged eIF4A1 RNA dependent ATPase activity expressed in Escherichia coli BL21(DE3) using single stranded poly(U) RNA as substrate after 40 mins by ADP-Glo luminescence assay
Assay data:3 Tested
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