BioAssay by Target (List) for Gene (Select 10886) - PubChem BioAssay - NCBI

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Select item 7187981.

Displacement of [3H]FFRF-NH2 from human flag-tagged NPFF2 receptor expressed in CHO cells at 5 uM by scintillation counting

Source:: ChEMBL

Protein Target:: Neuropeptide FF receptor 2

Assay data:73 Tested

AID:: 718798

Summary PubMed Citation Related BioAssays by Target

Select item 6979362.

Agonist activity at human NPFF2 receptor F5.34A mutant assessed as [3H]inositol phosphate accumulation at 10 uM after 2 hrs by liquid scintillation counting relative to NPFF

Source:: ChEMBL

Protein Target:: Neuropeptide FF receptor 2

Assay data:1 Tested

AID:: 697936

Summary PubMed Citation Related BioAssays by Target

Select item 6979373.

Ratio of EC50 for human NPFF2 receptor F5.34A mutant to EC50 for human wild type NPFF2 receptor

Source:: ChEMBL

Protein Target:: Neuropeptide FF receptor 2

Assay data:1 Tested

AID:: 697937

Summary PubMed Citation Related BioAssays by Target

Select item 6979384.

Agonist activity at human NPFF2 receptor F5.34A mutant assessed as [3H]inositol phosphate accumulation after 2 hrs by liquid scintillation counting

Source:: ChEMBL

Protein Target:: Neuropeptide FF receptor 2

Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested

AID:: 697938

Summary Compounds, Active Compounds, activity ≤ 1 µM PubMed Citation Related BioAssays by Target

Select item 6979395.

Agonist activity at human NPFF2 receptor Y5.38A mutant assessed as [3H]inositol phosphate accumulation at 10 uM after 2 hrs by liquid scintillation counting relative to NPFF

Source:: ChEMBL

Protein Target:: Neuropeptide FF receptor 2

Assay data:2 Tested

AID:: 697939

Summary PubMed Citation Related BioAssays by Target

Select item 6979406.

Ratio of EC50 for human NPFF2 receptor Y5.38A mutant to EC50 for human wild type NPFF2 receptor

Source:: ChEMBL

Protein Target:: Neuropeptide FF receptor 2

Assay data:1 Tested

AID:: 697940

Summary PubMed Citation Related BioAssays by Target

Select item 6979417.

Agonist activity at human NPFF2 receptor Y5.38A mutant assessed as [3H]inositol phosphate accumulation after 2 hrs by liquid scintillation counting

Source:: ChEMBL

Protein Target:: Neuropeptide FF receptor 2

Assay data:2 Active, 1 Activity ≤ 1 µM, 3 Tested

AID:: 697941

Summary Compounds, Active Compounds, activity ≤ 1 µM PubMed Citation Related BioAssays by Target

Select item 6979428.

Agonist activity at human NPFF2 receptor E5.27A mutant assessed as [3H]inositol phosphate accumulation at 10 uM after 2 hrs by liquid scintillation counting relative to NPFF

Source:: ChEMBL

Protein Target:: Neuropeptide FF receptor 2

Assay data:1 Tested

AID:: 697942

Summary PubMed Citation Related BioAssays by Target

Select item 6979439.

Ratio of EC50 for human NPFF2 receptor E5.27A mutant to EC50 for human wild type NPFF2 receptor

Source:: ChEMBL

Protein Target:: Neuropeptide FF receptor 2

Assay data:1 Tested

AID:: 697943

Summary PubMed Citation Related BioAssays by Target

Select item 69794410.

Agonist activity at human NPFF2 receptor E5.27A mutant assessed as [3H]inositol phosphate accumulation after 2 hrs by liquid scintillation counting

Source:: ChEMBL

Protein Target:: Neuropeptide FF receptor 2

Assay data:1 Active, 1 Activity ≤ 1 µM, 3 Tested

AID:: 697944

Summary Compounds, Active Compounds, activity ≤ 1 µM PubMed Citation Related BioAssays by Target

Select item 69809611.

Agonist activity at human NPFF2 receptor expressed in COS1 cells assessed as [3H]inositol phosphate accumulation at 10 uM after 2 hrs by liquid scintillation counting relative to NPFF

Source:: ChEMBL

Protein Target:: Neuropeptide FF receptor 2

Assay data:6 Tested

AID:: 698096

Summary PubMed Citation Related BioAssays by Target

Select item 69809712.

Agonist activity at human NPFF2 receptor expressed in COS1 cells assessed as [3H]inositol phosphate accumulation after 2 hrs by liquid scintillation counting

Source:: ChEMBL

Protein Target:: Neuropeptide FF receptor 2

Assay data:7 Active, 4 Activity ≤ 1 µM, 7 Tested

AID:: 698097

Summary Compounds, Active Compounds, activity ≤ 1 µM PubMed Citation Related BioAssays by Target

Select item 71879313.

Ratio of RF2 Ki to compound Ki for human flag-tagged NPFF2 receptor

Source:: ChEMBL

Protein Target:: Neuropeptide FF receptor 2

Assay data:1 Tested

AID:: 718793

Summary PubMed Citation Related BioAssays by Target

Select item 71879614.

Displacement of [3H]FFRF-NH2 from human flag-tagged NPFF2 receptor expressed in CHO cells by scintillation counting

Source:: ChEMBL

Protein Target:: Neuropeptide FF receptor 2

Assay data:24 Active, 21 Activity ≤ 1 µM, 24 Tested

AID:: 718796

Summary Compounds, Active Compounds, activity ≤ 1 µM PubMed Citation Related BioAssays by Target

Select item 71879715.

Displacement of [3H]FFRF-NH2 from human flag-tagged NPFF2 receptor expressed in CHO cells at 500 nM by scintillation counting

Source:: ChEMBL

Protein Target:: Neuropeptide FF receptor 2

Assay data:73 Tested

AID:: 718797

Summary PubMed Citation Related BioAssays by Target

Select item 69793516.

Agonist activity at human NPFF2 receptor D6.59A mutant assessed as [3H]inositol phosphate accumulation after 2 hrs by liquid scintillation counting

Source:: ChEMBL

Protein Target:: Neuropeptide FF receptor 2

Assay data:2 Active, 1 Activity ≤ 1 µM, 3 Tested

AID:: 697935

Summary Compounds, Active Compounds, activity ≤ 1 µM PubMed Citation Related BioAssays by Target

Select item 74312117.

Druggable Genome siRNA Screen for Enhancers of Camptothecin in MDA-MB-231 Breast Cancer Cells

Source:: National Center for Advancing Translational Sciences (NCATS)

Assay data:167 Active, 26449 Tested

AID:: 743121

Select item 74345418.

RNAi screen for vorinostat resistance genes - Primary Screen

Source:: Victorian Centre for Functional Genomics, Peter MacCallum Cancer Centre

Assay data:450 Active, 18118 Tested

AID:: 743454

Summary PubMed Citation

Select item 111728119.

A whole genome RNAi screen highlights components of the ER/Golgi as a prominent source of resistance to immunotoxin-mediated cytotoxicity

Source:: National Center for Advancing Translational Sciences (NCATS)

Assay data:555 Active, 64752 Tested

AID:: 1117281

Summary PubMed Citation

Select item 111735720.

InfectX: Pathogen infection siRNA screens

Source:: InfectX Consortium

Assay data:115372 Tested

AID:: 1117357

Summary PubMed Citation

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Target

RNAi Target, Active (6)
BioAssays with gene sequences specified as RNAi targets and the activity is active

BioActivity

Active Compounds (56)
BioAssays with compounds reported as biologically active
Activity (IC50, etc) ≤ 1 nM (9)
BioAssays with the active concentration of tested reagents less than 1 nanomolar
Activity (IC50, etc) ≤ 1 µM (48)
BioAssays with the active concentration of tested reagents less than 1 micromolar

Experiment Type

Summary (1)
BioAssays summarizing information from multiple assays for validated chemical probes or small molecule leads
Confirmatory (54)
Low-throughput BioAssays where the activity outcome is based on a dose-response relationship with multiple tested concentrations
Primary Screening (9)
Primary high-throughput BioAssays where the activity outcome is based on percentage inhibition from a single dose
Chemical Screens (100)
BioAssays reporting BioActivity of chemical reagents
RNAi Screens (13)
BioAssays reporting BioActivity of RNAi reagents

Assay Project (1)
BioAssays organized into assay projects based on linkage to Summary assays. Click an project below to retrieve the bioassay list for the project. Click 'All Assay Projects' to retrieve all assay projects represented by Summary assays.

Depositor Category

Literature Extraction (99)
BioAssays extracted from the literature
NIH MLP (1)
BioAssays deposited by the NIH Molecular Libraries Program
Other (13)
BioAssays deposited by other experimentalists

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