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ATPase, Na+/K+, Heart, Pig Eurofins Panlabs panel
Assay data:1 Tested
SummaryRelated BioAssays by Target
Inhibition of porcine pancreas alpha-amylase using CNP-G3 as substrate preincubated for 10 mins followed by substrate addition and measured after 30 mins by microplate reader analysis
SummaryPubMed Citation
Inhibition of porcine PDHc E1-subunit assessed as DCPIP reduction at 250 uM preincubated for 30 mins followed by pyruvate addition using 125 uM ThDP as substrate by microplate reader analysis
Assay data:12 Tested
SummaryPubMed CitationRelated BioAssays by Target
Ratio of Km for ThDP substrate activity at porcine PDHc E1-subunit to Ki for inhibition of porcine PDHc E1-subunit assessed as inhibition constant preincubated for 30 mins followed by pyruvate addition using 10 uM ThDP as substrate by microplate reader analysis
Assay data:3 Tested
Inhibition of porcine PDHc E1-subunit assessed as inhibition constant preincubated for 30 mins followed by pyruvate addition using 10 uM ThDP as substrate by microplate reader analysis
Assay data:3 Active, 3 Activity ≤ 1 µM, 3 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed CitationRelated BioAssays by Target
Inhibition of porcine PDHc E1-subunit assessed as DCPIP reduction preincubated for 30 mins followed by pyruvate addition using 10 uM ThDP as substrate by microplate reader analysis
Assay data:3 Active, 3 Tested
SummaryCompounds, ActivePubMed CitationRelated BioAssays by Target
Inhibition of porcine PDHc E1-subunit assessed as DCPIP reduction at 250 uM preincubated for 30 mins followed by pyruvate addition using 50 uM ThDP as substrate by microplate reader analysis
Assay data:32 Tested
Competitive inhibition of porcine pancreas elastase assessed as inhibition constant using N-succinyl-Ala-Ala-Ala-p-nitroanilide as substrate by Lineweaver-Burk plot analysis
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed Citation
Competitive inhibition of porcine pancreas elastase assessed as increase in Km with constant Vmax using N-succinyl-Ala-Ala-Ala-p-nitroanilide as substrate by Lineweaver-Burk plot analysis
Assay data:1 Active, 1 Tested
SummaryCompounds, ActivePubMed Citation
Potency index, ratio of oleanolic acid IC50 to test compound IC50 for inhibition of porcine pancreas elastase
Inhibition of porcine pancreas elastase preincubated with N-succinyl-Ala-Ala-Ala-p-nitroanilide substrate for 10 mins followed by enzyme addition and measured after 30 mins by microplate reader assay
Assay data:10 Active, 1 Activity ≤ 1 µM, 11 Tested
Cytotoxicity against pig LLC-PK1 cells
Assay data:2 Active, 2 Tested
Induction of pig brain microtubule stabilization at 25 uM measured after 15 mins by fluorescence based turbidimetry
Assay data:11 Tested
Inhibition of pig brain tubulin polymerization at 3 uM measured after 15 mins by fluorescence based turbidimetry
Inhibition of pig brain tubulin polymerization at 25 uM measured after 15 mins by fluorescence based turbidimetry
Assay data:8 Active, 10 Tested
Inhibition of porcine pancreatic alpha-amylase using starch as substrate incubated for 10 mins by absorbance based assay
Prodrug conversion in pig liver assessed as esterase-mediated Doxazolidine formation by measuring its half life at 1 uM by HPLC analysis
Inhibition of porcine kidney alpha/alpha-trehalase using p-nitrophenyl glycoside as substrate at 1000 uM by spectrometric method relative to control
Assay data:9 Tested
Inhibition of porcine kidney alpha/alpha-trehalase using p-nitrophenyl glycoside as substrate by spectrometric method
Assay data:2 Tested
Inhibition of porcine kidney alpha/alpha-trehalase
Assay data:1 Active, 2 Tested
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