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Inhibition of Thermotoga maritima membrane bound pyrophosphatase at 100 uM using sodium pyrophosphate as substrate preincubated for 5 mins followed by substrate addition and measured after 5 mins by spectrophotometry
Assay data:4 Active, 28 Tested
SummaryCompounds, ActivePubMed Citation
Inhibition of ATPgammaS-BODIPY binding to Thermotoga maritima His-tagged HK853 expressed in Escherichia coli BL21(DE3)pLysS Rosetta preincubated for 30 mins prior to ATPgammaS-BODIPY addition measured after 1 hr by coomassie staining-based SDS-PAGE analysis
Assay data:1 Active, 43 Tested
Inhibition of BODIPY-FL-ATPgammaS binding to Thermotoga maritima His-tagged HK853 expressed in Escherichia coli BL21(DE3)pLysS Rosetta after 25 mins by fluorescence polarization assay
Assay data:1 Active, 3 Tested
Inhibition of ADP-BODIPY binding to Thermotoga maritima HK853 after day 2 by fluorescence polarization assay
Assay data:1 Active, 1 Tested
Inhibition of ADP-BODIPY binding to Thermotoga maritima HK853 after day 1 by fluorescence polarization assay
Inhibition of Thermotoga maritima HK853 autophosphorylation using compound at non-aggregating concentration after 30 mins in presence of [gamma-33P]-ATP by phosphorescence-based assay
Assay data:7 Tested
SummaryPubMed Citation
Inhibition of ADP-BODIPY binding to Thermotoga maritima HK853 in presence of Triton X-100 by fluorescence polarization assay
Assay data:9 Tested
Inhibition of ADP-BODIPY binding to Thermotoga maritima HK853 by fluorescence polarization assay
Inhibition of Thermotoga maritima HK853 preincubated for 30 mins followed by B-ATPgammaS addition after 1 hr in dark by in-gel fluorescence analysis
Assay data:1 Active, 2 Tested
Inhibition of Thermotoga maritima histidine kinase HK853 autophosphorylation
Assay data:1 Active, 1 Activity ≤ 1 µM, 5 Tested
SummaryCompounds, ActiveCompounds, activity ≤ 1 µMPubMed Citation
Inhibition of Thermotoga maritima Beta-glucosidase GH1
Assay data:1 Active, 1 Activity ≤ 1 µM, 1 Tested
Activity of Thermotoga maritima S-adenosyl-L-homocysteine hydrolase Tm0172 assessed as homocysteine hydrolysis
Assay data:2 Tested
SummaryPubMed CitationRelated BioAssays by Target
Activity of Thermotoga maritima S-adenosyl-homocysteine deaminase Tm0936 assessed as ammonia production
Assay data:5 Tested
Inhibition of ATPgammaS-BODIPY binding to Thermotoga maritima His-tagged HK853 expressed in Escherichia coli BL21(DE3)pLysS Rosetta at 1.25 mM preincubated for 30 mins prior to ATPgammaS-BODIPY addition measured after 1 hr by coomassie staining-based SDS-PAGE analysis
Displacement of BODIPY-FL-ATP-gamma-S from Thermotoga maritima histidine kinase HK853 at 1 mM to 600 uM
Assay data:3 Active, 3 Tested
Inhibition of Thermotoga maritima histidine kinase HK853 autophosphorylation at 10 mM
Assay data:1 Tested
Induction of Thermotoga maritima histidine kinase HK853 aggregation up to 1000 uM by gel based assay
Inhibition of Thermotoga maritima histidine kinase HK853 autophosphorylation up to 10 mM
Mechanism based inhibition of Thermotoga maritima Sir2Tm assessed as longer-lived stabilized catalytic intermediate formation at 0.4 mM after 30 mins by MALDI-MS analysis in presence of NAD+
SummaryCompounds, ActiveRelated BioAssays by Target
Inhibition of Thermotoga maritima alpha-galactosidase
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