HGNC Approved Gene Symbol: ZNF185
Cytogenetic location: Xq28 Genomic coordinates (GRCh38): X:152,898,067-152,973,481 (from NCBI)
ZNF185 encodes a LIM-domain zinc finger protein. These proteins are thought to be involved in protein-protein interactions.
By exon trapping, Heiss et al. (1996) isolated a transcript on Xq28 corresponding to a cDNA with a LIM-domain zinc finger in the C terminus. Heiss et al. (1997) cloned a full-length ZNF185 cDNA encoding a deduced 307-amino acid protein containing a LIM domain preceded by a proline-rich region. The conserved cysteine and histidine residues that make up a LIM domain form a C2HC and a C4 motif, each of which coordinately binds one zinc ion (Sanchez-Garcia and Rabbitts, 1994). The ZNF185 protein shares 54% sequence identity with mouse Znf185. Northern blot analysis revealed expression of a 4.4-kb transcript in human adult prostate, testis, ovary, placenta, and peripheral blood as well as in embryonic kidney. In situ hybridization of mouse embryos revealed tissue- and stage-specific expression of Znf185, especially in differentiating connective tissue.
Medina et al. (2005) restored BRG1 (SMARCA4; 603254) in H1299 lung cancer cells and used cDNA microarray analysis to identify changes in gene expression. Forty-three transcripts became activated, whereas 2 were repressed. Both CYP3A4 (124010) and ZNF185 promoters recruited BRG1. The amount of ZNF185 transcript clearly varied among lung cancer cell lines and severely reduced levels were observed in BRG1-deficient cells, except A427 cells. In lung primary tumours, real-time RT-PCR revealed that 14 of 27 tumors had ZNF185 downregulation, respectively, when compared with normal lung. No significant correlation between reduced levels of BRG1 and ZNF185 was observed. Medina et al. (2005) concluded that transcriptional activation of ZNF185 and CYP3A4 is mediated by direct association of BRG1 with their promoters, and that a decreased level of ZNF185 is a common feature of lung tumors and may be of biologic relevance in lung carcinogenesis.
Heiss et al. (1997) determined that the ZNF185 gene spans about 59 kb of genomic DNA and contains 18 exons. The zinc finger motifs are encoded within exons 16 and 17.
By sequence analysis, Heiss et al. (1996) identified the ZNF185 gene in the DXS52 region of a transcript map of Xq28.
Heiss, N. S., Gloeckner, G., Bachner, D., Kioschis, P., Klauck, S. M., Hinzmann, B., Rosenthal, A., Herman, G. E., Poustka, A. Genomic structure of a novel LIM domain gene (ZNF185) in Xq28 and comparisons with the orthologous murine transcript. Genomics 43: 329-338, 1997. [PubMed: 9268636] [Full Text: https://doi.org/10.1006/geno.1997.4810]
Heiss, N. S., Rogner, U. C., Kioschis, P., Korn, B., Poustka, A. Transcriptional mapping in a 700-kb region around the DXS52 locus in Xq28: isolation of six novel transcripts and a novel ATPase isoform (hPMCA5). Genome Res. 6: 478-491, 1996. [PubMed: 8828037] [Full Text: https://doi.org/10.1101/gr.6.6.478]
Medina, P. P., Carretero, J., Ballestar, E., Angulo, B., Lopez-Rios, F., Esteller, M., Sanchez-Cespedes, M. Transcriptional targets of the chromatin-remodelling factor SMARCA4/BRG1 in lung cancer cells. Hum. Molec. Genet. 14: 973-982, 2005. [PubMed: 15731117] [Full Text: https://doi.org/10.1093/hmg/ddi091]
Sanchez-Garcia, I., Rabbitts, T. H. The LIM domain: a new structural motif found in zinc-finger-like proteins. Trends Genet. 10: 315-320, 1994. [PubMed: 7974745] [Full Text: https://doi.org/10.1016/0168-9525(94)90034-5]