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KRAS : methods and protocols
- Author(s):
- Stephen, Andrew G, editor
- Esposito, Dominic, (Biochemist), editor
- Title(s):
- KRAS : methods and protocols / edited by Andrew G. Stephen and Dominic Esposito.
- Series:
- Methods in molecular biology, ; 2797 ISSN 1064-3745
Springer protocols
- Country of Publication:
- United States
- Publisher:
- New York, NY : Humana Press, [2024]
- Copyright Date:
- 2024
- Description:
- xi, 365 pages : illustrations (some color), charts ; 26 cm.
-
Language:
- English
- ISBN:
- 9781071638217
1071638211
9781071638224
107163822X
- Electronic Links:
- https://doi.org/10.1007/978-1-0716-3822-4
- Summary:
- "This volume details protocols ranging from high yield production metabolically labeled KRAS for NMR studies to approaches that quantify engagement of novel molecules that bind KRAS in live cells. Chapters focus on protein production and characterization, biochemical assays, cell-based assays, KRAS-membrane interactions, targeting KRAS, and cell models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, KRAS: Methods and Protocols aims to provide methods that will be instrumental in the development of future clinically approved KRAS therapeutics"--Back cover.
- MeSH:
- Genes, ras*
Kirsten murine sarcoma virus
ras Proteins
- Publication Type(s):
- Laboratory Manual
- Contents:
- KRAS as a therapeutic target -- The abundance of KRAS and RAS gene mutations in cancer -- Production of isotopically labeled KRAS4b -- Nucleotide Exchange on RAS Proteins using Hydrolysable and Non-Hydrolysable Nucleotides -- Crystallographic studies of KRAS in complex with small molecules and RAS-binding proteins / Albert H. Chan and Dhirendra K. Simanshu -- Considerations around structure-based drug discovery for KRAS using DOCK / Mayukh Chakrabarti, Y. Stanley Tan, and Trent E. Balius -- Measurement of KRAS GTPase activity / Dana Rabara and Andrew G. Stephen -- Affinity Measurement of Non-covalent Interactions of the covalent KRAS G12C GDP inhibitor MRTX849 to RAS isoforms using surface plasmon resonance / Partick Alexander and Andrew G. Stephen -- 1D and 2D NMR for KRAS:ligand binding / Gabriel Cornilescu -- Luminescence based techniques for KRAS thermal stability monitoring / Kari Kopra -- MALDI-TOF mass spectrometry-based assay for measuring covalent target engagement of KRAS G12C inhibitors / Marcin Dyba, John-Paul Denson, and Anna E. Maciag -- KRAS4b:RAF-1 homogenous time-resolved fluorescence resonance energy transfer assay for drug discovery / Erik K. Larsen, Maria Abreu-Blanco, Dana Rabara, and Andrew G. Stephen -- Biophysical characterization of RAS-SOS Complexes by Native Mass Spectrometry / Sangho Yun, Elena Scott, and Arthur Laganowsky -- Profiling complex RAS-effector interactions using NMR spectroscopy / Regina Strakhova and Matthew J. Smith -- Probing RAS Function Using Monobody and NanoBiT Technologies / Michael Whaby, Rakesh Sathish Nair, and John P. O'Bryan -- Analysis of the guanine nucleotide-bound state of KRAS by ion-pair reversed-phase high-performance liquid chromatography / Makoto Araki and Kenji Kontani -- Real-time monitoring of RAS activity using in vitro and in-cell NMR spectroscopy / Qingci Zhao, Ichio Shimada, and Noritaka Nishida -- Studying RAS Interactions in live cells with BRET / John Columbus and Thomas Turbyville -- FLIM-FRET Protein-Protein Interaction Assay / Pedro Andrade Bonilla and Rebika Shrestha -- High-throughput cell-based screening of small molecule KRAS signaling inhibitors using a homogeneous time resolved fluorescence (HTRF) assay / Brian P. Smith, Megan Rigby, Roger Ma, and Anna E. Maciag -- A method to conditionally measure target engagement at intracellular RAS and RAF complexes / James D. Vasta, Ani Michaud, J. Aaron Crapster, and Mathew B. Roberts -- FLAG-KRAS4B as a model system for KRAS4B proteoform and PTM evaluation by mass spectrometry / Robert A. D'Ippolito, Grace M. Scheidemantle, Brian P. Smith, Katie Powell, Scott Eury, Abigail Neish, Jennifer Mehalko, Lauren Beaumont, Nicole Fer, Vanessa Wall, William Burgan, Anna E. Maciag, Dominic Esposito, and Caroline J. DeHart -- Creation of an isogenic H/N/KRAS-less mouse embryonic fibroblast cell line panel derived from a size sorted diploid clone / William Burgen -- Quality control of an Isogenic H/N/KRAS-less mouse embryonic fibroblast cell line panel / Bingfang Xu and Katie Powell -- A facile method to append a Bio-ID tag to endogenous mutant Kras alleles / Konstantin Budagyan, Alexa C. Cannon, and Johnathan Chernoff.
- Other ID:
- (OCoLC)1435943278
- NLM ID:
- 9918863350806676 [Book]