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Enzyme engineering and evolution. Specific enzyme applications

Author(s):
Tawfik, Dan S, editor
Title(s):
Enzyme engineering and evolution. Specific enzyme applications / edited by Dan S. Tawfik.
Other Title(s):
Specific enzyme applications
Series:
Methods in enzymology, ; volume 644 ISSN 0076-6879
Edition:
First edition.
Country of Publication:
United States
Publisher:
Cambridge, MA : Academic Press, 2020.
Copyright Date:
2020
Description:
xvi, 296 pages : illustrations (some color) ; 24 cm.
Language:
English
ISBN:
9780128244319
0128244313
9780128244326
MeSH:
Biomedical Engineering
Enzymes/chemical synthesis*
Evolution, Molecular
Publication Type(s):
Laboratory Manual
Notes:
Includes bibliographical references.
Contents:
1. Engineering lytic polysaccharide monooxygenases (LPMOs) / Zarah Forsberg, Anton A. Stepnov, Guro Kruge Nærdal, Geir Klinkenberg and Vincent G. H. Eijsink -- 2. Strategies for the expression and characterization of artificial myoglobin-based carbene transferases / Daniela M. Carminati, Eric J. Moore and Rudi Fasan -- 3. Methods for the detection and analysis of dioxygenase catalyzed dihydroxylation in mutant derived libraries / Julian L. Wissner, Wendy Escobedo-Hinojosa, Peter M. Heinemann, Andreas Hunold and Bernhard Hauer -- 4. Enhancing promiscuous chemistries of a Schiff-base forming enzyme by divergent evolution / Duncan S. Macdonald and Donald Hilvert -- 5. Glutamine-walking: Creating reactive substrates for transglutaminase-mediated protein labeling / Lukas Deweid, Adem Hadjabdelhafid-Parisien, Kiana Lafontaine, Léa N.C. Rochet, Harald Kolmar and Joelle N. Pelletier -- 6. Engineering aldolases for asymmetric synthesis / Mikael Widersten -- 7. Ultra-high throughput screening for novel protease specificities / Eirini Rousounelou, Steven Schmitt, Luzius Pestalozzi, Martin Held, Tania M. Roberts and Sven Panke -- 8. Directed evolution of transcriptional switches using dual-selector systems / Yuki Kimura and Daisuke Umeno -- 9. Methods for competitive enrichment and evaluation of superior DNA ligases / Janine K. Sharma, Luke J. Stevenson, Katherine J. Robins, Adele K. Williamson, Wayne M. Patrick and David F. Ackerley -- 10. Directed evolution of custom polymerases using droplet microfluidics / Derek Vallejo, Ali Nikoomanzar and John C. Chaput -- 11. Optically guided mass spectrometry to screen microbial colonies for directed enzyme evolution / Pu Xue, Tong Si and Huimin Zhao -- 12. Split enzymes: Design principles and strategy / Shion A. Lim and James A. Wells.
Other ID:
(OCoLC)1163955502
NLM ID:
9918350786506676 [Book]

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