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YAC protocols

Author(s):
MacKenzie, Alasdair
Title(s):
YAC protocols / edited by Alasdair MacKenzie.
Other Title(s):
Yeast artificial chromosome protocols
Series:
Methods in molecular biology, ; 349 ISSN 1064-3745
Edition:
2nd ed.
Country of Publication:
United States
Publisher:
Totowa, N.J. : Humana Press, c2006.
Description:
xv, 203 p. : ill.
Language:
English
ISBN:
9781588296122 (alk. paper)
1588296121 (alk. paper)
1597451584 (e-book)
LCCN:
2006000445
Electronic Links:
https://link.springer.com/book/10.1385/1597451584
MeSH:
Chromosomes, Artificial, Yeast*
Genetic Techniques
Genetic Vectors*
Publication Type(s):
Laboratory Manual
Notes:
Includes bibliographical references and index.
Also issued online.
Contents:
Markers, selection, and media in yeast artificial chromosomes cloning / David Markie -- Construction of yeast artificial chromosome libraries from pathogens and nonmodel organisms / Cecilia P. Sanchez and Michael Lanzer -- Analysis and screening of yeast artificial chromosome libraries by filter hybridization / Cecilia P. Sanchez and Michael Lanzer -- Analysis of Yeast Artificial Chromosome DNA by restriction digestion, southern blotting nucleic acid hybridization and polymerase chain reaction / Sylvia Vasiliou and John Peter Quinn -- Postgenomic bioinformatic analysis of yeast artificial chromosome sequence / Kerry Miller, Scott Davidson, and Alasdair MacKenzie -- Transformation of yeast using bioactive beads with surface-immobilized yeast artificial chromosomes / Shigeki Kawakami ... [et al.] --
Modification and amplification of yeast artificial chromosomes / Sanbing Shen, Anthony Harmar, and Nick Hastie -- Modifying yeast artificial chromosomes to generate Cre/LoxP and FLP/FRT site-specific deletions and inversions / Gabriela G. Loots -- Selective isolation of large chromosomal regions by transformation-associated recombination cloning for structural and functional analysis of mammalian genomes / Natalay Kouprina, Vladimir N. Noskov, and Vladimir Larionov -- A polymerase chain reaction-mediated yeast artificial chromosome-splitting technology for generating targeted yeast artificial chromosomes subclones / Yeon-Hee Kim ... [et al.] -- Genomic reconstruction by serial mitotic recombination of yeast artificial chromosomes / David Markie, Emma Jones, and Jiannis Ragoussis -- Targeting vector construction by yeast artificial chromosome modification / Peter J. Murray --
Production of yeast artificial chromosomes transgenic mice by pronuclear injection of one-cell embryos / Alasdair MacKenzie -- Generation of yeast artificial chromosomes transgenic mice by intracytoplasmic sperm injection / Pedro N. Moreira ... [et al.] -- Establishment of cell lines that exhibit correct ontogenic stage-specific gene expression profiles from tissues of yeast artificial chromosome transgenic mice using chemically induced growth signals / C. Anthony Blau and Kenneth R. Peterson -- Application of yeast artificial chromosomes in fluorescence in situ hybridization / Thomas Liehr -- Contribution of yeast artificial chromosome-based physical maps to the final assembly of the trypanosoma cruzi genome / Marcia R.M. Santos ... [et al.].
Other ID:
(OCoLC)62885444
NLM ID:
101264909 [Book]

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