Chromatin Immunoprecipitation

A technique for identifying specific DNA sequences that are bound, in vivo, to proteins of interest. It involves formaldehyde fixation of CHROMATIN to crosslink the DNA-BINDING PROTEINS to the DNA. After shearing the DNA into small fragments, specific DNA-protein complexes are isolated by immunoprecipitation with protein-specific ANTIBODIES. Then, the DNA isolated from the complex can be identified by PCR amplification and sequencing.

Year introduced: 2005

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Tree Number(s): E05.393.170, E05.478.605.160

MeSH Unique ID: D047369

Entry Terms:

• Immunoprecipitation, Chromatin

Previous Indexing:

• Precipitin Tests (2000-2004)

All MeSH Categories
Analytical, Diagnostic and Therapeutic Techniques and Equipment Category
Investigative Techniques
Genetic Techniques
Chromatin Immunoprecipitation
Chromatin Immunoprecipitation Sequencing

All MeSH Categories
Analytical, Diagnostic and Therapeutic Techniques and Equipment Category
Investigative Techniques
Immunologic Techniques
Immunoprecipitation
Chromatin Immunoprecipitation
Chromatin Immunoprecipitation Sequencing