Ligase Chain Reaction

A DNA amplification technique based upon the ligation of OLIGONUCLEOTIDE PROBES. The probes are designed to exactly match two adjacent sequences of a specific target DNA. The chain reaction is repeated in three steps in the presence of excess probe: (1) heat denaturation of double-stranded DNA, (2) annealing of probes to target DNA, and (3) joining of the probes by thermostable DNA ligase. After the reaction is repeated for 20-30 cycles the production of ligated probe is measured.

Year introduced: 2001

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Tree Number(s): E05.393.620.311

MeSH Unique ID: D022521

Entry Terms:

• Chain Reaction, Ligase
• Chain Reactions, Ligase
• Ligase Chain Reactions
• Reaction, Ligase Chain
• Reactions, Ligase Chain

Previous Indexing:

• Gene Amplification (1980-2000)

See Also:

• Polymerase Chain Reaction

All MeSH Categories
Analytical, Diagnostic and Therapeutic Techniques and Equipment Category
Investigative Techniques
Genetic Techniques
Nucleic Acid Amplification Techniques
Ligase Chain Reaction