Microspectrophotometry

Analytical technique for studying substances present at enzyme concentrations in single cells, in situ, by measuring light absorption. Light from a tungsten strip lamp or xenon arc dispersed by a grating monochromator illuminates the optical system of a microscope. The absorbance of light is measured (in nanometers) by comparing the difference between the image of the sample and a reference image.

Year introduced: 1990

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Tree Number(s): E05.196.712.726.300, E05.196.867.826.300

MeSH Unique ID: D015591

Entry Terms:

• Microspectrophotometries

Previous Indexing:

• Spectrophotometry (1966-1989)

All MeSH Categories
Analytical, Diagnostic and Therapeutic Techniques and Equipment Category
Investigative Techniques
Chemistry Techniques, Analytical
Photometry
Spectrophotometry
Microspectrophotometry

All MeSH Categories
Analytical, Diagnostic and Therapeutic Techniques and Equipment Category
Investigative Techniques
Chemistry Techniques, Analytical
Spectrum Analysis
Spectrophotometry
Microspectrophotometry