nuclease - MeSH

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Select item 680232821.

Techniques for measuring specific nucleic acid interaction with another nucleic acid or with a protein by digestion of the non-interacting nucleic acid by various nucleases. After all non-interacting regions are eliminated by nuclease digestion, the protected nucleic acid that remains is analyzed. DNA FOOTPRINTING utilizes this technique to analyze the DNA contact sites of DNA-BINDING PROTEINS.

Year introduced: 2001

Select item 680088362.

Micrococcal Nuclease

An enzyme that catalyzes the endonucleolytic cleavage to 3'-phosphomononucleotide and 3'-phospholigonucleotide end-products. It can cause hydrolysis of double- or single-stranded DNA or RNA. (From Enzyme Nomenclature, 1992) EC 3.1.31.1.

Year introduced: 1973(1965)

Select item 670218353.

Serratia marcescens nuclease [Supplementary Concept]

formerly EC 3.1.4.9; acts on DNA, RNA and double-stranded homopolymers such as poly I-C; has been sequenced, Genbank M19495

Date introduced: January 1, 1976

Select item 680455854.

Flap Endonucleases

Endonucleases that remove 5' DNA sequences from a DNA structure called a DNA flap. The DNA flap structure occurs in double-stranded DNA containing a single-stranded break where the 5' portion of the downstream strand is too long and overlaps the 3' end of the upstream strand. Flap endonucleases cleave the downstream strand of the overlap flap structure precisely after the first base-paired nucleotide, creating a ligatable nick.

Year introduced: 2004

Select item 670669645.

viral alkaline nuclease [Supplementary Concept]

has both exo and endonuclease activity

Date introduced: February 25, 1991

Select item 20234826.

Zinc Finger Nucleases

Genetically engineered nucleases that cleave DNA at a defined distance from specific DNA sequences recognized by ZINC FINGER DNA-BINDING DOMAINS. They are composed of a DNA cleaving domain adapted from DNA endonucleases fused to a zinc finger DNA-binding domain.

Year introduced: 2018

Select item 671195137.

nuclease I, Syncephalastrum racemosum [Supplementary Concept]

sugar-non-specific nuclease from a member of the Mucorales; amino acid sequence in first source; GenBank AF043515

Date introduced: June 22, 1999

Select item 671045748.

staphylococcal nuclease R [Supplementary Concept]

an analog of micrococcal nuclease (staphylococcal nuclease A) in which a hexapeptide (DPTVYS) is attached to the N-terminal alanine of micrococcal nuclease

Date introduced: March 18, 1997

Select item 680347439.

RNA-Induced Silencing Complex

A multicomponent, ribonucleoprotein complex comprised of one of the family of ARGONAUTE PROTEINS and the guide strand of the one of the 20- to 30-nucleotide small RNAs. RISC cleaves specific RNAs, which are targeted for degradation by homology to these small RNAs. Functions in regulating gene expression are determined by the specific argonaute protein and small RNA including siRNA (RNA, SMALL INTERFERING), miRNA (MICRORNA), or piRNA (PIWI-INTERACTING RNA).

Year introduced: 2003

Select item 6708510810.

APEX1 protein, human [Supplementary Concept]

RefSeq NM_001641

Date introduced: February 2, 1994

Select item 6706240111.

poly(A)-specific ribonuclease [Supplementary Concept]

bifunctional enzyme responsible for both poly(A)-polymerizing & poly(A)-hydrolyzing activities; removes the poly(A) tail as the first step in degrading mRNA; RefSeq NM_002582 (human)

Date introduced: February 15, 1990

Select item 6705298912.

nucA protein, Bacillus subtilis [Supplementary Concept]

MW 75kDA; from membranes of competent Bacillus subtilis cells; shows DNA-binding & nuclease activity; believed to be involved in entry of DNA into competent B subtilis cells

Date introduced: August 24, 1987

Select item 6703638513.

endodeoxyribonuclease uvrABC [Supplementary Concept]

from E coli; requires ATP & Mg(2+); removes ultraviolet-damaged bases

Date introduced: December 17, 1982

Select item 6702387114.

nuclease H [Supplementary Concept]

extracellular halophilic nuclease from Micrococcus varians, subspecies halophilis; degrades RNA to 5'-ribonucleotides

Date introduced: May 8, 1980

Select item 202788715.

CRISPR-Associated Protein 9

An RNA-guided endodeoxyribonuclease that associates with CRISPR SEQUENCES in STREPTOCOCCUS PYOGENES and other bacteria where it participates in an adaptive immune function to cleave foreign DNA complimentary to small GUIDE RNA (sgRNAs). Structurally, Cas9 consists of an ALPHA-HELIX module and a nuclease module connected by a single helix. The nuclease module contains two enzymatic domains: RuvC, which cleaves non-target DNA strand, and an HNH nuclease domain, which cleaves the target strand. Specificity for the DNA target depends on the presence of a protospacer adjacent motif (PAM) sequence, a 2-6 nucleotide DNA sequence immediately following the sequence targeted by Cas9.

Year introduced: 2019

Select item 200134216.

Fanconi anemia-associated nuclease 1, S pombe [Supplementary Concept]

a nuclease

Date introduced: July 14, 2014

Select item 6752610117.

Mung Bean Nuclease [Supplementary Concept]

Date introduced: July 29, 2008

Select item 6752609818.

Endonuclease S1, Aspergillus [Supplementary Concept]

GenBank D45902

Date introduced: July 29, 2008