DROSHA drosha ribonuclease III [Homo sapiens (human)] - Gene

Summary

Official Symbol: DROSHAprovided by HGNC
Official Full Name: drosha ribonuclease IIIprovided by HGNC
Primary source: HGNC:HGNC:17904
See related: Ensembl:ENSG00000113360 MIM:608828; AllianceGenome:HGNC:17904
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Homo sapiens
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo
Also known as: RN3; ETOHI2; RNASEN; RANSE3L; RNASE3L; HSA242976
Summary: This gene encodes a ribonuclease (RNase) III double-stranded RNA-specific ribonuclease and subunit of the microprocessor protein complex, which catalyzes the initial processing step of microRNA (miRNA) synthesis. The encoded protein cleaves the stem loop structure from the primary microRNA (pri-miRNA) in the nucleus, yielding the precursor miRNA (pre-miRNA), which is then exported to the cytoplasm for further processing. In a human cell line lacking a functional copy of this gene, canonical miRNA synthesis is reduced. Somatic mutations in this gene have been observed in human patients with kidney cancer. [provided by RefSeq, Sep 2016]
Expression: Ubiquitous expression in brain (RPKM 9.5), ovary (RPKM 7.6) and 25 other tissues See more
Orthologs: mouse all
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Genomic context

Location:: 5p13.3

Exon count:: 36

Annotation release	Status	Assembly	Chr	Location
RS_2024_08	current	GRCh38.p14 (GCF_000001405.40)	5	NC_000005.10 (31400494..31532093, complement)
RS_2024_08	current	T2T-CHM13v2.0 (GCF_009914755.1)	5	NC_060929.1 (31514128..31645804, complement)
RS_2024_09	previous assembly	GRCh37.p13 (GCF_000001405.25)	5	NC_000005.9 (31400601..31532200, complement)

Chromosome 5 - NC_000005.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: HPA RNA-seq normal tissues HPA RNA-seq normal tissues
Description: RNA-seq was performed of tissue samples from 95 human individuals representing 27 different tissues in order to determine tissue-specificity of all protein-coding genes
BioProject: PRJEB4337
Publication: PMID 24309898
Analysis date: Wed Apr 4 07:08:55 2018

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cytoplasmic and nuclear DROSHA in human villous trophoblasts.
Title: Cytoplasmic and nuclear DROSHA in human villous trophoblasts.
The 3' UTR polymorphisms rs3742330 in DICER1 and rs10719 in DROSHA genes are not associated with primary open-angle and angle-closure glaucoma: As case-control study.
Title: The 3' UTR polymorphisms rs3742330 in DICER1 and rs10719 in DROSHA genes are not associated with primary open-angle and angle-closure glaucoma: As case-control study.
Downregulation of DROSHA: Could It Affect miRNA Biogenesis in Endometriotic Menstrual Blood Mesenchymal Stem Cells?
Title: Downregulation of DROSHA: Could It Affect miRNA Biogenesis in Endometriotic Menstrual Blood Mesenchymal Stem Cells?
Association of DROSHA rs6877842, rs642321 and rs10719 polymorphisms with increased susceptibility to breast cancer: A case-control study with genotype and haplotype analysis.
Title: Association of DROSHA rs6877842, rs642321 and rs10719 polymorphisms with increased susceptibility to breast cancer: A case-control study with genotype and haplotype analysis.
Immunohistochemical expression of Drosha is reduced in eutopic and ectopic endometrium of women with adenomyosis.
Title: Immunohistochemical expression of Drosha is reduced in eutopic and ectopic endometrium of women with adenomyosis.
The Drosha-Independent MicroRNA6778-5p/GSK3beta Axis Mediates the Proliferation of Gastric Cancer Cells.
Title: The Drosha-Independent MicroRNA6778-5p/GSK3β Axis Mediates the Proliferation of Gastric Cancer Cells.
The microRNA processor DROSHA is a candidate gene for a severe progressive neurological disorder.
Title: The microRNA processor DROSHA is a candidate gene for a severe progressive neurological disorder.
The impact of Dicer, Drosha, and Exportin-5 levels in polycystic ovary syndrome (PCOS) diagnosis and phenotyping.
Title: The impact of Dicer, Drosha, and Exportin-5 levels in polycystic ovary syndrome (PCOS) diagnosis and phenotyping.
p38 MAPK-mediated loss of nuclear RNase III enzyme Drosha underlies amyloid beta-induced neuronal stress in Alzheimer's disease.
Title: p38 MAPK-mediated loss of nuclear RNase III enzyme Drosha underlies amyloid beta-induced neuronal stress in Alzheimer's disease.
Sequencing of Argonaute-bound microRNA/mRNA hybrids reveals regulation of the unfolded protein response by microRNA-320a.
Title: Sequencing of Argonaute-bound microRNA/mRNA hybrids reveals regulation of the unfolded protein response by microRNA-320a.

Submit: New GeneRIF Correction See all GeneRIFs (156)

Phenotypes

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Find tests for this gene in the NIH Genetic Testing Registry (GTR)

Review eQTL and phenotype association data in this region using PheGenI

Variation

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See variants in ClinVar

See studies and variants in dbVar

See Variation Viewer (GRCh37.p13)

See Variation Viewer (GRCh38)

HIV-1 interactions

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Protein interactions

Protein	Gene	Interaction	Pubs
Pr55(Gag)	gag	Knockdown of Drosha by shRNA leads to inhibit enrichment of HIV-1 Gag in intracellular vacuoles, but enhance viral particle release in Gag expressing cells	PubMed

Go to the HIV-1, Human Interaction Database

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D5S2192E (e-PCR)
- UniSTS:154398

RH78996 (e-PCR)
- UniSTS:47651

EST20D9 (e-PCR)
- UniSTS:263218

D8S2279 (e-PCR)
- UniSTS:473907

SHGC-143335 (e-PCR)
- UniSTS:171864

SHGC-79713 (e-PCR)
- UniSTS:101219

G63617 (e-PCR)
- UniSTS:140728

SHGC-154556 (e-PCR)
- UniSTS:178134

RH92866 (e-PCR)
- UniSTS:86234

Gene Ontology Provided by GOA

Function	Evidence Code	Pubs
enables DEAD/H-box RNA helicase binding	IPI Inferred from Physical Interaction more info	PubMed
enables R-SMAD binding	IPI Inferred from Physical Interaction more info	PubMed
enables RNA binding	HDA more info	PubMed
enables RNA binding	IPI Inferred from Physical Interaction more info	PubMed
enables SMAD binding	IPI Inferred from Physical Interaction more info	PubMed
enables lipopolysaccharide binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables primary miRNA binding	IPI Inferred from Physical Interaction more info	PubMed
enables primary miRNA binding	NAS Non-traceable Author Statement more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein homodimerization activity	IDA Inferred from Direct Assay more info	PubMed
enables ribonuclease III activity	IBA Inferred from Biological aspect of Ancestor more info
enables ribonuclease III activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in defense response to Gram-negative bacterium	IDA Inferred from Direct Assay more info	PubMed
involved_in defense response to Gram-positive bacterium	IDA Inferred from Direct Assay more info	PubMed
involved_in miRNA metabolic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of gene expression	IEA Inferred from Electronic Annotation more info
involved_in pre-miRNA processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in primary miRNA processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in primary miRNA processing	IDA Inferred from Direct Assay more info	PubMed
involved_in primary miRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in rRNA processing	IEA Inferred from Electronic Annotation more info
involved_in regulation of inflammatory response	IEA Inferred from Electronic Annotation more info
involved_in regulation of miRNA metabolic process	IEA Inferred from Electronic Annotation more info
involved_in regulation of regulatory T cell differentiation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytosol	IDA Inferred from Direct Assay more info
located_in glutamatergic synapse	IEA Inferred from Electronic Annotation more info
part_of microprocessor complex	IBA Inferred from Biological aspect of Ancestor more info
part_of microprocessor complex	IDA Inferred from Direct Assay more info	PubMed
part_of microprocessor complex	IPI Inferred from Physical Interaction more info	PubMed
located_in nucleolus	IEA Inferred from Electronic Annotation more info
located_in nucleoplasm	IDA Inferred from Direct Assay more info
located_in nucleoplasm	TAS Traceable Author Statement more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in postsynaptic density	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: ribonuclease 3

Names: RNase III; drosha, double-stranded RNA-specific endoribonuclease; nuclear RNase III Drosha; p241; putative protein p241 which interacts with transcription factor Sp1; putative ribonuclease III; ribonuclease type III, nuclear

NP_001093882.1

EC 3.1.26.3

NP_001369437.1

EC 3.1.26.3

NP_037367.3

EC 3.1.26.3

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

Genomic

NG_051574.1 RefSeqGene

Range

5083..136682

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001100412.2 → NP_001093882.1 ribonuclease 3 isoform 2

See identical proteins and their annotated locations for NP_001093882.1

Status: REVIEWED

Description

Transcript Variant: This variant (2) differs in the 5' UTR and lacks an alternate in-frame exon in the 5' coding region, compared to variant 1. The resulting protein (isoform 2) is shorter than isoform 1.

Source sequence(s)

AA917625, AC008768, BC024261, BX647724, DB065470

Consensus CDS

CCDS47194.1

Related

ENSP00000424161.1, ENST00000513349.5

Conserved Domains (3) summary

PRK00102
Location:1068 → 1296

rnc; ribonuclease III; Reviewed

cd00593
Location:922 → 1042

RIBOc; Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in post-transcriptional ...

TIGR01642
Location:217 → 344

U2AF_lg; U2 snRNP auxilliary factor, large subunit, splicing factor
NM_001382508.1 → NP_001369437.1 ribonuclease 3 isoform 1

Status: REVIEWED

Description

Transcript Variant: Variants 1 and 3 encode the same isoform (1).

Source sequence(s)

AC008768, AC022417, AC106802

Consensus CDS

CCDS47195.1

UniProtKB/Swiss-Prot

E7EMP9, Q7Z5V2, Q86YH0, Q9NRR4, Q9NW73, Q9Y2V9, Q9Y4Y0

Related

ENSP00000339845.3, ENST00000344624.8

Conserved Domains (3) summary

PRK00102
Location:1105 → 1333

rnc; ribonuclease III; Reviewed

cd00593
Location:959 → 1079

RIBOc; Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in post-transcriptional ...

TIGR01642
Location:217 → 317

U2AF_lg; U2 snRNP auxilliary factor, large subunit, splicing factor
NM_013235.5 → NP_037367.3 ribonuclease 3 isoform 1

See identical proteins and their annotated locations for NP_037367.3

Status: REVIEWED

Description

Transcript Variant: This variant (1) encodes the longer isoform (1). Variants 1 and 3 encode the same isoform.

Source sequence(s)

AA917625, AC008768, AF189011, BC024261, DB065470

Consensus CDS

CCDS47195.1

UniProtKB/Swiss-Prot

E7EMP9, Q7Z5V2, Q86YH0, Q9NRR4, Q9NW73, Q9Y2V9, Q9Y4Y0

Related

ENSP00000425979.2, ENST00000511367.6

Conserved Domains (3) summary

PRK00102
Location:1105 → 1333

rnc; ribonuclease III; Reviewed

cd00593
Location:959 → 1079

RIBOc; Ribonuclease III C terminal domain. This group consists of eukaryotic, bacterial and archeal ribonuclease III (RNAse III) proteins. RNAse III is a double stranded RNA-specific endonuclease. Prokaryotic RNAse III is important in post-transcriptional ...

TIGR01642
Location:217 → 317

U2AF_lg; U2 snRNP auxilliary factor, large subunit, splicing factor