Plod3 procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Plod3provided by MGI
Official Full Name: procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3provided by MGI
Primary source: MGI:MGI:1347008
See related: Ensembl:ENSMUSG00000004846 AllianceGenome:MGI:1347008
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: LH3
Summary: Enables procollagen glucosyltransferase activity and procollagen-lysine 5-dioxygenase activity. Involved in hydroxylysine biosynthetic process and peptidyl-lysine hydroxylation. Acts upstream of or within several processes, including chordate embryonic development; endothelial cell morphogenesis; and extracellular matrix organization. Located in several cellular components, including endoplasmic reticulum; extracellular space; and trans-Golgi network. Is expressed in several structures, including branchial arch; ear; future brain; incisor; and skeleton. Orthologous to human PLOD3 (procollagen-lysine,2-oxoglutarate 5-dioxygenase 3). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Ubiquitous expression in subcutaneous fat pad adult (RPKM 39.0), adrenal adult (RPKM 31.2) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 5 G2; 5 76.09 cM

Exon count:: 19

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	5	NC_000071.7 (137015873..137025500)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	5	NC_000071.6 (136987019..136996646)

Chromosome 5 - NC_000071.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

results indicate that LH3 overexpression attenuates susceptibility to hypertensive intracerebral hemorrhage
Title: Overexpression of LH3 reduces the incidence of hypertensive intracerebral hemorrhage in mice.
Data indicate that lysyl hydroxylase 3 is essential for the glucosyltion of mannan-binding lectin MBL-A.
Title: Lysyl hydroxylase 3 modifies lysine residues to facilitate oligomerization of mannan-binding lectin.
LH3 is an important regulator of adiponectin biosynthesis, secretion and activity.
Title: The activities of lysyl hydroxylase 3 (LH3) regulate the amount and oligomerization status of adiponectin.
LH3-mediated glucosylation occurs at the specific molecular loci in the type I collagen molecule and plays critical roles in controlling collagen cross-linking, fibrillogenesis, and mineralization.
Title: Lysyl hydroxylase 3-mediated glucosylation in type I collagen: molecular loci and biological significance.
the major function of LH3 in osteoblasts is to glucosylate galactosylhydroxylysine residues in type I collagen and that an impairment of this LH3 function significantly affects type I collagen fibrillogenesis.
Title: Lysyl hydroxylase 3 glucosylates galactosylhydroxylysine residues in type I collagen in osteoblast culture.
Results report an important role for lysyl hydroxylase 3 in the organization of the extracellular matrix (ECM) and cytoskeleton.
Title: Reduction of lysyl hydroxylase 3 causes deleterious changes in the deposition and organization of extracellular matrix.
LH3 has lysyl hydroxylase and galactosylhydroxylysyl glucosyltransferase T activities in vivo, and is essential for the formation of the basement membrane
Title: Glycosylation catalyzed by lysyl hydroxylase 3 is essential for basement membranes.
results demonstrate that LH3 is indispensable for biosynthesis of type IV collagen and for basement membrane stability during early development and that loss of LH3's functions leads to embryonic lethality
Title: Premature aggregation of type IV collagen and early lethality in lysyl hydroxylase 3 null mice.
LH3 is present and active in the extracellular space
Title: Lysyl hydroxylase 3 (LH3) modifies proteins in the extracellular space, a novel mechanism for matrix remodeling.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (1) 1 citation
Endonuclease-mediated (1)
Gene trapped (1)
Targeted (6) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables L-ascorbic acid binding	IEA Inferred from Electronic Annotation more info
enables iron ion binding	IEA Inferred from Electronic Annotation more info
enables iron ion binding	ISO Inferred from Sequence Orthology more info
enables metal ion binding	ISO Inferred from Sequence Orthology more info
enables procollagen galactosyltransferase activity	ISO Inferred from Sequence Orthology more info
enables procollagen galactosyltransferase activity	ISS Inferred from Sequence or Structural Similarity more info
enables procollagen glucosyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables procollagen glucosyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables procollagen glucosyltransferase activity	ISO Inferred from Sequence Orthology more info
enables procollagen-lysine 5-dioxygenase activity	IBA Inferred from Biological aspect of Ancestor more info
enables procollagen-lysine 5-dioxygenase activity	IDA Inferred from Direct Assay more info	PubMed
enables procollagen-lysine 5-dioxygenase activity	ISO Inferred from Sequence Orthology more info
enables small molecule binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within basement membrane assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in collagen fibril organization	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within collagen fibril organization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within collagen metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within endothelial cell morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within epidermis morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in hydroxylysine biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within in utero embryonic development	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within lung morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within neural tube development	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peptidyl-lysine hydroxylation	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within peptidyl-lysine hydroxylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in peptidyl-lysine hydroxylation	ISO Inferred from Sequence Orthology more info
involved_in peptidyl-lysine hydroxylation	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein O-linked glycosylation	IEA Inferred from Electronic Annotation more info
involved_in protein O-linked glycosylation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within protein localization	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within vasodilation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in Golgi apparatus	IBA Inferred from Biological aspect of Ancestor more info
located_in Golgi apparatus	ISO Inferred from Sequence Orthology more info	PubMed
located_in collagen-containing extracellular matrix	HDA more info	PubMed
is_active_in collagen-containing extracellular matrix	IBA Inferred from Biological aspect of Ancestor more info
located_in collagen-containing extracellular matrix	IDA Inferred from Direct Assay more info	PubMed
is_active_in endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum	ISO Inferred from Sequence Orthology more info
located_in endoplasmic reticulum lumen	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
is_active_in extracellular space	IBA Inferred from Biological aspect of Ancestor more info
located_in extracellular space	IDA Inferred from Direct Assay more info	PubMed
located_in rough endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in trans-Golgi network	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3

Names: lysyl hydroxylase 2; lysyl hydroxylase 3

NP_036092.1

EC 1.14.11.4

EC 2.4.1.50

EC 2.4.1.66

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_011962.3 → NP_036092.1 multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3 precursor

Status: VALIDATED

Source sequence(s)

BB692664, BC054734

Consensus CDS

CCDS19759.1

UniProtKB/Swiss-Prot

Q542E0, Q9CYY9, Q9R0E1

Related

ENSMUSP00000004968.5, ENSMUST00000004968.11

Conserved Domains (1) summary

smart00702
Location:576 → 739

P4Hc; Prolyl 4-hydroxylase alpha subunit homologues